Department of Cell Biology, Physiology and Immunology, Faculty of Biology, University of Barcelona, 08028 Barcelona, Spain
Department of Cell Biology, Physiology and Immunology, Faculty of Biology, University of Barcelona, 08028 Barcelona, Spain.
J Exp Biol. 2019 Apr 15;222(Pt 8):jeb195925. doi: 10.1242/jeb.195925.
Fish skin mucus is proposed as a novel target for the study of physiological condition and to conduct minimally invasive monitoring of fish. Whereas mucus composition has been a major interest of recent studies, no practical techniques have been proposed to gain understanding of the capacity and rhythm of production and exudation. Here, we used stable isotope analysis (SIA) with a labelled meal, packaged in gelatine capsules, to evaluate mucus production and renewal in a fish model, the gilthead sea bream (). Mucus C- and N-enrichment reached higher levels at 12 h post-ingestion without significant differences at 24 h. When the formation of new mucus was induced, C-enrichment in the new mucus doubled whereas N-enrichment only increased by 10%. These results indicate the feasibility of adopting SIA in mucus studies and allow us to propose this methodology as a means to improve knowledge of mucus turnover in fish and other animals.
鱼皮黏液被提议作为研究生理状况和进行鱼类微创监测的新目标。尽管黏液组成一直是最近研究的主要关注点，但尚未提出任何实用技术来了解其产生和渗出的能力和节律。在这里，我们使用稳定同位素分析 (SIA) 与标记餐一起，用明胶胶囊包装，以评估鱼类模型（金头鲷）的黏液产生和更新。在摄入后 12 小时，C 和 N 同位素在黏液中的富集达到较高水平，而在 24 小时时没有显著差异。当诱导新黏液形成时，新黏液中的 C 同位素富集增加了一倍，而 N 同位素富集仅增加了 10%。这些结果表明 SIA 在黏液研究中的可行性，并使我们能够提出这种方法来提高对鱼类和其他动物黏液周转率的认识。
