Department of Laboratory Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China.
Department of Thyroid & Breast Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China.
Dis Markers. 2019 Mar 3;2019:6149381. doi: 10.1155/2019/6149381. eCollection 2019.
Secreted frizzled-related protein 2 (sFRP2) is a secreted protein associated with cancer drug resistance and metastasis. However, few studies have reported serum sFRP2 levels in breast cancer. We evaluated serum sFRP2 as a potential biomarker for breast cancer.
Serum sFRP2 concentrations were detected in 274 breast cancer patients along with 147 normal healthy controls by enzyme-linked immunosorbent assay (ELISA). Diagnostic significance was evaluated by area under the curve (AUC) analysis and the Youden index. Prognostic significance was determined by Kaplan-Meier survival method and univariate and multivariate Cox proportional hazard regression model analyses.
Serum sFRP2 was elevated in breast cancer patients compared to normal healthy controls ( < 0.001). The sensitivity of sFRP2 in diagnosing breast cancer was 76.9% at a specificity of 76.6%. Elevated serum sFRP2 levels are associated with primary tumor size, TNM stage, and lymph node metastases. The Kaplan-Meier curves showed a significant association of serum sFRP2 with progression-free survival. The multivariate Cox analysis confirmed that high serum sFRP2 was an independent prognostic factor for poor prognosis (HR = 3.89, 95% CI = 1.95-7.68, = 0.001).
In conclusion, serum sFRP2 may serve as a potential biomarker for breast cancer diagnosis and prognostic evaluation.
分泌型卷曲相关蛋白 2（sFRP2）是一种与癌症药物耐药性和转移相关的分泌蛋白。然而，很少有研究报道乳腺癌患者的血清 sFRP2 水平。我们评估了血清 sFRP2 作为乳腺癌潜在生物标志物的作用。
采用酶联免疫吸附试验（ELISA）检测 274 例乳腺癌患者和 147 例正常健康对照者的血清 sFRP2 浓度。通过曲线下面积（AUC）分析和 Youden 指数评估诊断意义。通过 Kaplan-Meier 生存法和单因素及多因素 Cox 比例风险回归模型分析评估预后意义。
与正常健康对照者相比，乳腺癌患者的血清 sFRP2 水平升高（<0.001）。sFRP2 诊断乳腺癌的敏感性为 76.9%，特异性为 76.6%。血清 sFRP2 水平升高与原发肿瘤大小、TNM 分期和淋巴结转移有关。Kaplan-Meier 曲线显示血清 sFRP2 与无进展生存期显著相关。多因素 Cox 分析证实，高血清 sFRP2 是预后不良的独立预后因素（HR=3.89，95%CI=1.95-7.68，=0.001）。
总之，血清 sFRP2 可能是一种用于乳腺癌诊断和预后评估的潜在生物标志物。
