Qiu Yunhua, Zheng Jinzhou, Yang Jianfeng, Li Feng, Zhou Xiqiu, Song Xiaoyun
Department of General Surgery, Pudong Branch of Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, P.R. China.
Iran J Basic Med Sci. 2019 Jan;22(1):86-92. doi: 10.22038/ijbms.2018.33173.7922.
This study was aimed to evaluate whether single nucleotide polymorphisms (SNPs) of TLR4 and common living habits of prostate hyperplasia (BPH) patients would affect the subjects' risk and prognosis.
We totally recruited 501 BPH patients and 964 healthy controls. The patients' international prostate symptom score (IPSS) and quality of life assessment (QoL) were designated as the prognostic indexes for BPH patients. Altogether 7 SNPs within TLR4 were selected, and the interactions among SNPs and living habits were explained with multi-factor dimensionality reduction (MDR) modeling.
The mutant alleles of rs10983755 (G>A) and rs1927907 (G>A) tended to put on risk of BPH, yet the wide alleles of rs4986791 (C>T) and rs115336889 (G>C) were associated with incremental susceptibility to BPH (<0.05). The rs10983755 (GA) and rs1927907 (GA) were suggested as the marker of non-aggressive BPH, whereas rs4986791 (TT) could symbolize aggressive BPH (<0.05). The homozygotes of rs4986791 (TT) and rs115336889 (CC) could improve the IPSS change, and rs115336889 (CC) was also correlated with more obviously ameliorated Qol change (<0.05). Finally, MDR modeling suggested that rs4986791 (TT) and rs115336889 (GG) shaped the genotyping combination featured by the lowest risk of BPH when smoking or drinking history was also evaluated.
The SNPs situated within TLR4 were potent candidates for predicting risk and prognosis of BPH patients, and their interactions within environmental parameters also helped to develop effective strategies for preventing and treating BPH.
本研究旨在评估Toll样受体4（TLR4）的单核苷酸多态性（SNP）以及前列腺增生（BPH）患者的常见生活习惯是否会影响受试者的风险和预后。
我们共招募了501例BPH患者和964例健康对照者。将患者的国际前列腺症状评分（IPSS）和生活质量评估（QoL）指定为BPH患者的预后指标。共选择了TLR4基因内的7个SNP，并采用多因素降维（MDR）模型解释SNP与生活习惯之间的相互作用。
rs10983755（G>A）和rs1927907（G>A）的突变等位基因倾向于增加BPH的风险，而rs4986791（C>T）和rs115336889（G>C）的野生型等位基因与BPH易感性增加相关（<0.05）。rs10983755（GA）和rs1927907（GA）被认为是非侵袭性BPH的标志物，而rs4986791（TT）可能代表侵袭性BPH（<0.05）。rs4986791（TT）和rs115336889（CC）的纯合子可改善IPSS变化，rs115336889（CC）也与更明显改善的QoL变化相关（<0.05）。最后，MDR模型表明，当同时评估吸烟或饮酒史时，rs4986791（TT）和rs115336889（GG）形成了BPH风险最低的基因分型组合。
位于TLR4基因内的SNP是预测BPH患者风险和预后的有力候选指标，它们在环境参数中的相互作用也有助于制定有效的BPH防治策略。
