受银纳米颗粒影响的L.的体外生长。
In vitro growth of L. affected by silver nanoparticles.
作者信息
Timoteo Caroline de Oliveira, Paiva Renato, Dos Reis Michele Valquíria, Claro Pedro Ivo Cunha, Ferraz Luthiane Machado, Marconcini Jose Manoel, de Oliveira Juliano Elvis
机构信息
1Department of Biology, Sector of Plant physiology, Federal University of Lavras, Lavras, MG Brazil.
2Graduate Program in Materials Science and Engineering (PPG-CEM), Federal University of São Carlos, São Carlos, SP Brazil.
出版信息
3 Biotech. 2019 Apr;9(4):145. doi: 10.1007/s13205-019-1674-z. Epub 2019 Mar 21.
The effect of silver nanoparticles (AgNPs) on plant cells, since their phytotoxicity potential is not yet fully understood. In this context, the aim of the present study was to elucidate the effects of AgNPs in the in vitro culture of . For this purpose, seeds were grown in MS medium supplemented with different concentrations of AgNPs. Growth and development of seedlings were evaluated through germination, seedling size and biomass and biochemical and anatomical analyses. At the end of 60 days of cultivation, it was observed that the in vitro germination of this species is not affected by the presence of AgNPs and that at low concentrations (0.385 mg L) it can promote an increase in seedlings biomass. However, higher concentration (15.4 mg L) leads to a reduction in seedling size and root system, but no changes were observed in the seedlings antioxidant metabolism and anatomy. These results demonstrate that the phytotoxicity of AgNPs in is related to the concentration of nanoparticles to which the specie is exposed.
银纳米颗粒（AgNPs）对植物细胞的影响，因为其潜在的植物毒性尚未完全了解。在此背景下，本研究的目的是阐明AgNPs在[植物名称未给出]体外培养中的作用。为此，将[植物名称未给出]种子在添加了不同浓度AgNPs的MS培养基中培养。通过发芽、幼苗大小和生物量以及生化和解剖分析来评估幼苗的生长和发育。在培养60天后，观察到该物种的体外发芽不受AgNPs存在的影响，并且在低浓度（0.385毫克/升）下它可以促进幼苗生物量的增加。然而，较高浓度（15.4毫克/升）会导致幼苗大小和根系减少，但在幼苗的抗氧化代谢和解剖结构方面未观察到变化。这些结果表明，AgNPs对[植物名称未给出]的植物毒性与该物种接触的纳米颗粒浓度有关。
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