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3
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Sci Rep. 2017 Jan 24;7:41214. doi: 10.1038/srep41214.
4
Laccase Gene Family in Cerrena sp. HYB07: Sequences, Heterologous Expression and Transcriptional Analysis.蜡蘑属真菌HYB07中的漆酶基因家族：序列、异源表达及转录分析
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6
High Level Secretion of Laccase (LccH) from a Newly Isolated White-Rot Basidiomycete, Hexagonia hirta MSF2.新分离的白腐担子菌Hexagonia hirta MSF2中漆酶（LccH）的高水平分泌
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7
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9
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10
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担子菌Bm-2漆酶基因的分子特征及5'非翻译区（5'UTR）分析

Molecular characterization of laccase genes from the basidiomycete Bm-2 and analysis of the 5' untranslated region (5'UTR).

作者信息

Pereira-Patrón Alejandrina, Solis-Pereira Sara, Lizama-Uc Gabriel, Ramírez-Prado Jorge H, Pérez-Brito Daisy, Tapia-Tussell Raul

机构信息

1Depto. de Ingeniería Química y Bioquímica, Tecnológico Nacional de México, Instituto Tecnológico de Mérida, Av. Tecnológico Km 4.5 S/N, 97118 Mérida, Yucatán Mexico.

2Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, Calle 43 No. 130 x 32 y 34, Col. Chuburná de Hidalgo, 97205 Mérida, Yucatán Mexico.

出版信息

3 Biotech. 2019 Apr;9(4):160. doi: 10.1007/s13205-019-1691-y. Epub 2019 Mar 30.

DOI:10.1007/s13205-019-1691-y
PMID:30944807
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6441420/
Abstract

The aim of this study was to identify and characterize laccase genes produced by Bm-2 in a liquid medium, both with and without induction. The amplification of 5'and 3'regions of laccase sequences was obtained by the RACE-PCR method, and these were assembled to obtain a cDNA of total length. Two new laccase genes were isolated from basal medium (-) and lignocellulosic grapefruit substrate (-), both encoding open reading frames of 2566 bp. Both laccase-predicted proteins consisted of 521 amino acids, four copper-binding regions, a signal peptide, and five potential glycosilation sites (Asn-Xaa-Ser/Tre). Moreover, the deduced amino acid sequences share about 76-85% identity with other laccases of WRF. Sequence comparison showed 47 synonymous point mutations between - and -. In addition, 5' untranslated regions (UTR) of laccase genes - and - showed differences in length and number of regulatory elements that may affect transcriptional or translational expression of these genes.

摘要

本研究的目的是鉴定和表征Bm-2在液体培养基中产生的漆酶基因，包括有无诱导的情况。通过RACE-PCR方法获得漆酶序列5'和3'区域的扩增产物，并将其组装以获得全长cDNA。从基础培养基（-）和木质纤维素葡萄柚底物（-）中分离出两个新的漆酶基因，它们都编码2566 bp的开放阅读框。预测的两种漆酶蛋白均由521个氨基酸、四个铜结合区域、一个信号肽和五个潜在的糖基化位点（Asn-Xaa-Ser/Thr）组成。此外，推导的氨基酸序列与WRF的其他漆酶具有约76-85%的同一性。序列比较显示-和-之间有47个同义点突变。此外，漆酶基因-和-的5'非翻译区（UTR）在长度和调控元件数量上存在差异，这可能会影响这些基因的转录或翻译表达。