Fukami Y, Owada M K, Sumi M, Hayashi F
Biochem Biophys Res Commun. 1986 Sep 14;139(2):473-9. doi: 10.1016/s0006-291x(86)80015-8.
We have isolated a tyrosine-specific protein kinase from the acetylcholine receptor (AChR)-rich membranes of the electric ray Narke japonica. The enzyme is immunologically related to p60v-src, the product of the transforming gene of Rous sarcoma virus. A substantial phosphatidylinositol (PI) kinase activity was associated with this enzyme when it was purified through tyrosine-agarose affinity chromatography used previously for the purification of p60v-src. However, by subsequent chromatography on casein-agarose, most of the associated PI kinase activity was separated from the tyrosine kinase activity. The results suggest that the tyrosine-specific protein kinase in the AChR-rich membranes of N. japonica has no intrinsic PI kinase activity.
我们从日本电鳐富含乙酰胆碱受体（AChR）的膜中分离出了一种酪氨酸特异性蛋白激酶。该酶在免疫上与Rous肉瘤病毒转化基因的产物p60v-src相关。当通过先前用于纯化p60v-src的酪氨酸琼脂糖亲和层析对该酶进行纯化时，一种大量的磷脂酰肌醇（PI）激酶活性与该酶相关。然而，通过随后在酪蛋白琼脂糖上的层析，大部分相关的PI激酶活性与酪氨酸激酶活性分离。结果表明，日本电鳐富含AChR的膜中的酪氨酸特异性蛋白激酶没有内在的PI激酶活性。
