Sayhan O, Ozdemirli M, Nurten R, Bermek E
Biochem Biophys Res Commun. 1986 Sep 30;139(3):1210-4. doi: 10.1016/s0006-291x(86)80306-0.
A cellular ADP-ribosyltransferase, specific for elongation factor 2 (EF-2), is found in extracts from rat liver. Co-migrating with EF-2 throughout purification, this activity is, moreover, located in the protein bands corresponding to EF-2 after native or sodium dodecyl sulfate polyacrylamide gel electrophoresis. The observed activity is thus implicated to be an inherent property of EF-2. Preincubation of EF-2 with GuoPPCH2Pox inhibits endogenous, but not diphtheria toxin catalyzed ADP-ribosylation.
