[电针对坐骨神经损伤大鼠坐骨神经及腰脊髓组织中肌肉萎缩和Slit/Robo信号通路的影响]

[Effect of electroacupuncture on muscular atrophy and Slit/Robo signaling in sciatic nerve and lumbar spinal cord tissues in rats with sciatic nerve injury].

作者信息

Du Xu, Wang Peng-Li, Yan Shu-Guang, Liu Juan, Yin Qian, Li Ya-Ya

机构信息

Department of Acupuncture-moxibustion and Tuina, Shaanxi University of Traditional Chinese Medicine, Xianyang 712046, Shaanxi Province, China.

The Affiliated Hospital, Shaanxi University of Traditional Chinese Medicine, Xianyang 712046, Shaanxi Province, China.

出版信息

Zhen Ci Yan Jiu. 2019 Feb 25;44(2):102-6. doi: 10.13702/j.1000-0607.180186.

DOI:10.13702/j.1000-0607.180186

PMID:30945485

Abstract

OBJECTIVE

To observe the effect of electroacupuncture (EA) of "Huantiao"(GB30) and" Zusanli"(ST36)on muscular atrophy and expression of Slit-Robo GTPase-activating protein(srGAP)1, 2 and 3 in the injured sciatic nerve and lumbar spinal cord tissues in sciatic nerve injury (SNI) rats, so as to reveal its mechanisms underlying improvement of peripheral nerve injury (PNI)．.

METHODS

A total of 120 healthy male SD rats were randomly divided into control, sham-operation, model and EA groups (＝30 rats in each) which were further divided into 7, 15 and 23 d subgroups (＝10 rats in each subgroup). The SNI model was established by transecting the right sciatic nerve beneath the piriformis and immediately subsequent end-to-end suture. Rats of the sham operation group received an incision of the corresponding skin and suture. EA (5 Hz/20 Hz, 2－3 mA) was applied to the right GB30 and ST36 for 15 min, once daily, 6 days a week separately for 1，2 and 3 weeks. Rats in the sham-operation and model groups were grasped in the similar procedure as the EA group. The wet weight of gastrocnemius muscles (WWG) on both sides was measured to calculate the recovery rate (weight of the right WWG/weight of the left WWG×100%), and the expression levels of srGAP1, srGAP2 and srGAP3 proteins in the sciatic nerve and the spinal cord (L4-L6) tissues were detected by Western blot.

RESULTS

After modeling and compared with the control and sham-operation groups, the recovery rate of WWG was significantly reduced, and the expression levels of srGAP1, srGAP2 and srGAP3 proteins of the sciatic nerve and lumbar spinal cord on day 7, 15 and 23 were considerably increased in the model group (<0.01). Following the EA treatment, the reco-very rate of WWG was obviously increased and the expression levels of srGAP1, srGAP2 and srGAP3 proteins of both sciatic nerve and spinal cord on day 7, 15 and 23 were further significantly up-regulated in the EA group relevant to the model group (<0.05，<0.01). In addition, the expression levels of the 3 proteins in both sciatic nerve and lumbar spinal cord peaked on day 15 and attenuated on day 23.

CONCLUSION

EA of GB30 and ST36 may relieve gastrocnemius atrophy in SNI rats, which is related to its function in up-regulating the Slit/Robo signaling in the sciatic nerve and lumbar spinal cord to promote the axonal targeting regeneration and repair of axonal plasma nutrition transportation.

摘要

目的

观察电针“环跳”（GB30）和“足三里”（ST36）对坐骨神经损伤（SNI）大鼠损伤坐骨神经及腰脊髓组织中肌肉萎缩及Slit-Robo GTP酶激活蛋白（srGAP）1、2和3表达的影响，以揭示其改善周围神经损伤（PNI）的机制。

方法

将120只健康雄性SD大鼠随机分为对照组、假手术组、模型组和电针组（每组30只），每组再分为7、15和23天亚组（每组10只）。通过在梨状肌下方横断右侧坐骨神经并立即进行端端缝合建立SNI模型。假手术组大鼠进行相应皮肤切口和缝合。将电针（5Hz/20Hz，2 - 3mA）施加于右侧GB30和ST36，每次15分钟，每天1次，每周6天，分别持续1、2和3周。假手术组和模型组大鼠以与电针组相似的方式抓取。测量双侧腓肠肌湿重（WWG）以计算恢复率（右侧WWG重量/左侧WWG重量×100%），并通过蛋白质免疫印迹法检测坐骨神经和脊髓（L4 - L6）组织中srGAP1、srGAP2和srGAP3蛋白的表达水平。

结果

建模后，与对照组和假手术组相比，模型组WWG恢复率显著降低，且在第7、15和23天坐骨神经和腰脊髓中srGAP1、srGAP2和srGAP3蛋白的表达水平显著升高（P<0.01）。电针治疗后，电针组WWG恢复率明显升高，且在第7、15和23天坐骨神经和脊髓中srGAP1、srGAP2和srGAP3蛋白的表达水平相对于模型组进一步显著上调（P<0.05，P<0.01）。此外，坐骨神经和腰脊髓中这3种蛋白的表达水平在第15天达到峰值，在第23天减弱。