Nicholson A W, Frankfort H M, Davis N G, Ferrari S, Lamb R A, Robertson H D
Biochim Biophys Acta. 1986 Nov 13;868(2-3):153-63. doi: 10.1016/0167-4781(86)90018-7.
The NS1 mRNA of the influenza A virus WSN (H0N1) strain was isolated from a cell-free transcription system, and from the cytoplasm of virus-infected HeLa cells. The 32P-labeled NS1 mRNA derived from the infected cell cytoplasm was characterized by the secondary enzymatic analysis of sixteen of its large or distinct RNAase T1-resistant oligonucleotides. Several WSN strain-specific nucleotide differences from the previously-determined sequence of NS1 mRNA from the PR8 (H0N1) strain of influenza A virus, were located within these sequences. The RNAase T1-resistant oligonucleotides were placed within the primary sequence of NS1 mRNA, using the PR8 strain sequence data. The resulting linear map was then used to identify NS2 mRNA isolated from the infected cell cytoplasm, and an NS-related RNA species generated from NS1 mRNA incubated in a HeLa cell-free extract.
甲型流感病毒WSN（H0N1）株的NS1 mRNA是从无细胞转录系统以及病毒感染的HeLa细胞的细胞质中分离出来的。源自受感染细胞细胞质的32P标记的NS1 mRNA通过对其16个大的或不同的抗RNA酶T1寡核苷酸进行二级酶分析来表征。与先前确定的甲型流感病毒PR8（H0N1）株NS1 mRNA序列相比，几个WSN株特异性核苷酸差异位于这些序列内。利用PR8株序列数据，将抗RNA酶T1寡核苷酸置于NS1 mRNA的一级序列中。然后，所得的线性图谱用于鉴定从受感染细胞细胞质中分离出的NS2 mRNA，以及在HeLa无细胞提取物中孵育的NS1 mRNA产生的一种NS相关RNA种类。
