Department of Medicine, Division of Infectious Diseases, Saint Louis University School of Medicine, St. Louis, Missouri, United States of America.
The Emmes Corporation, Rockville, Maryland, United States of America.
PLoS One. 2019 Apr 4;14(4):e0214467. doi: 10.1371/journal.pone.0214467. eCollection 2019.
Preventive viral vaccine efficacy trials require large-scale sample analysis to quantitate immune responses and their correlation with infection outcomes. Traditional plaque reduction assays measure a functionally important form of humoral immunity, neutralizing antibody titer. These assays, however, are time-consuming and laborious. We previously developed a higher throughput assay of neutralizing antibody to herpes simplex viruses 1 and 2 (Blevins et al., PLOS ONE, 10(12), e0144738) using the enzyme-linked virus inducible system (ELVIS) cell line; this cell line produces β-galactosidase in response to HSV infection. Here, serum samples from recipients of an investigational vaccine in the Herpevac Trial for Women were used to compare the ELVIS cell assay with the lower throughput, traditional plaque reduction assay. We demonstrate that neutralizing antibody titers to HSV-1 or HSV-2 determined using ELVIS cells positively correlate with neutralizing antibody titers determined by traditional plaque reduction assay, thus validating a higher throughput alternative for large-scale sample analysis.
预防病毒疫苗的疗效试验需要大规模的样本分析来量化免疫反应及其与感染结果的相关性。传统的噬菌斑减少测定法测量一种具有重要功能的体液免疫，即中和抗体滴度。然而，这些测定法耗时费力。我们之前开发了一种更高通量的单纯疱疹病毒 1 和 2 的中和抗体测定法（Blevins 等人，PLOS ONE，10（12），e0144738），使用酶联病毒诱导系统（ELVIS）细胞系；该细胞系在受到 HSV 感染时产生β-半乳糖苷酶。在这里，我们使用来自 Herpevac 女性疫苗试验的受种者的血清样本，将 ELVIS 细胞测定法与较低通量的传统噬菌斑减少测定法进行比较。我们证明，使用 ELVIS 细胞测定的 HSV-1 或 HSV-2 中和抗体滴度与传统噬菌斑减少测定法测定的中和抗体滴度呈正相关，从而验证了一种用于大规模样本分析的高通量替代方法。
