College of Agronomy, Henan Agricultural University, Zhengzhou, 450002, China.
State Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, 450002, China.
Biol Res. 2019 Apr 4;52(1):19. doi: 10.1186/s40659-019-0228-5.
Recent studies indicate that circular RNAs (circRNAs) may play important roles in the regulation of plant growth and development. Plant roots are the main organs of nutrient and water uptake. However, whether circRNAs involved in the regulation of plant root growth remains to be elucidated.
LH9, XN979 and YN29 are three Chinese wheat varieties with contrasting root lengths. Here, the root circRNA expression profiles of LH9, XN979 and YN29 were examined by using high-throughput sequencing technology.
Thirty-three and twenty-two differentially expressed circRNAs (DECs) were identified in the YN29-LH9 comparison and YN29-XN979 comparison, respectively. Among them, ten DECs coexisted in both comparisons. As the roots of both LH9 and XN979 were significantly larger and deeper than YN29, the ten DECs coexisting in the two comparisons were highly likely to be involved in the regulation of wheat root length. Moreover, three of the ten DECs have potential miRNA binding sites. Real-time PCR analysis showed that the expression levels of the potential binding miRNAs exhibited significant differences between the long root plants and the short root plants.
The expression levels of some circRNAs exhibited significant differences in wheat varieties with contrasting root phenotypes. Ten DECs involved in the regulation of wheat root length were successfully identified in which three of them have potential miRNAs binding sites. The expression levels of putative circRNA-binding miRNAs were correlated with their corresponding circRNAs. Our results provide new clues for studying the potential roles of circRNAs in the regulation of wheat root length.
最近的研究表明，环状 RNA（circRNA）可能在植物生长和发育的调控中发挥重要作用。植物根是吸收养分和水分的主要器官。然而，参与植物根生长调控的 circRNA 尚待阐明。
LH9、XN979 和 YN29 是三个具有不同根长的中国小麦品种。在这里，我们利用高通量测序技术检测了 LH9、XN979 和 YN29 的根 circRNA 表达谱。
在 YN29-LH9 比较和 YN29-XN979 比较中分别鉴定出 33 个和 22 个差异表达的 circRNA（DECs）。其中，10 个 DECs 同时存在于两个比较中。由于 LH9 和 XN979 的根明显大于和深于 YN29，因此这两个比较中存在的 10 个 DECs 很可能参与了小麦根长的调控。此外，这 10 个 DECs 中的三个具有潜在的 miRNA 结合位点。实时 PCR 分析显示，长根植物和短根植物之间潜在结合 miRNA 的表达水平存在显著差异。
在具有不同根表型的小麦品种中，一些 circRNA 的表达水平存在显著差异。成功鉴定出参与调控小麦根长的 10 个 DECs，其中 3 个具有潜在的 miRNA 结合位点。推测的 circRNA 结合 miRNA 的表达水平与其相应的 circRNA 相关。我们的研究结果为研究 circRNA 在调控小麦根长中的潜在作用提供了新线索。
