University of Chemical Technology and Metallurgy, Department of Biotechnology, 1756 Sofia, 8 St. Kliment Ohridski Blvd., Bulgaria.
University of Chemical Technology and Metallurgy, Department of Fundamentals of Chemical Technology, 1756 Sofia, 8 St. Kliment Ohridski Blvd., Bulgaria.
Mater Sci Eng C Mater Biol Appl. 2019 Jul;100:608-615. doi: 10.1016/j.msec.2019.03.018. Epub 2019 Mar 6.
A series of Zn-doped hybrid materials based on silica from tetraethoxysilane (TEOS) and hydroxypropyl cellulose (HPC) were prepared by a sol-gel route. The structure, morphology and thermal behavior of synthesized hybrids were characterized by infrared (IR) spectroscopy, ultraviolet-visible spectroscopy (UV-Vis), transmission electron microscopy (TEM) and differential thermal analysis with thermogravimetric analysis (DTA/TG). The obtained materials were investigated for a potential biomedical application. The antibacterial properties of hybrids were investigated by measuring the inhibition zones formed around the materials containing different zinc content in presence of reference strains of Gram-positive and Gram-negative bacteria. The biocompatibility tests showed no cytotoxicity and genotoxicity, as well as no changes in actin cytoskeleton organization for hybrids with Zn content below 5 wt%.
采用溶胶-凝胶法制备了一系列基于正硅酸乙酯（TEOS）和羟丙基纤维素（HPC）的掺锌杂化材料。通过红外（IR）光谱、紫外-可见光谱（UV-Vis）、透射电子显微镜（TEM）和差示热分析与热重分析（DTA/TG）对合成杂化材料的结构、形态和热行为进行了表征。研究了所得材料在潜在的生物医学应用中的性能。通过测量含有不同锌含量的材料在革兰氏阳性和革兰氏阴性细菌标准菌株存在下形成的抑菌圈，研究了杂化材料的抗菌性能。细胞毒性和遗传毒性试验表明，锌含量低于 5wt%的杂化材料没有细胞毒性和遗传毒性，也没有改变肌动蛋白细胞骨架的组织。
