Yun Bo-Young, Cho Chunghee, Cho Byung-Nam
Department of Life Science, The Catholic University of Korea, Bucheon, Korea.
Department of Life Science Gwangju Institute of Science and Technology, Gwangju, Korea.
Anim Cells Syst (Seoul). 2019 Feb 20;23(2):135-142. doi: 10.1080/19768354.2018.1554543. eCollection 2019 Apr.
The 16K isoform of rat prolactin (16K rPRL) performs multiple functions in various systems including angiogenesis, tumorigenesis, and reproduction. Recently, 16K rPRL has attained prominence as a possible therapeutic target in pathophysiological conditions. However, the integral function and mechanism of 16K rPRL in various systems has not been elucidated. To this end, a transient gain-of-function animal model was adopted. An expression DNA plasmid containing 16K rPRL or rPRL gene was introduced into the muscle of adult mice by direct injection. The mRNA and protein expression levels of 16K rPRL were detected by initial RT-PCR and subsequent Southern blot and western blot, respectively. When the expression vector was introduced, the results were as follows: First, 16K rPRL combined with rPRL reduced angiogenesis in the testis whereas rPRL alone induced angiogenesis. Second, 16K rPRL combined with rPRL reduced WBC proliferation, whereas rPRL alone increased WBC proliferation. Third, 16K rPRL combined with rPRL reduced diestrus, whereas rPRL alone extended diestrus. Fourth, 16K rPRL combined with rPRL unexpectedly increased testosterone (T) levels, whereas rPRL alone did not increase T levels. Taken together, our data suggest that the 16K rPRL isoform performs integral functions in angiogenesis in the testis, WBC proliferation, and reproduction, although the action of 16K rPRL is not always antagonistic.
大鼠催乳素的16K异构体（16K rPRL）在包括血管生成、肿瘤发生和生殖在内的各种系统中发挥多种功能。最近，16K rPRL作为病理生理条件下可能的治疗靶点而备受关注。然而，16K rPRL在各种系统中的整体功能和机制尚未阐明。为此，采用了一种瞬时功能获得性动物模型。通过直接注射将含有16K rPRL或rPRL基因的表达DNA质粒导入成年小鼠的肌肉中。分别通过初始RT-PCR以及随后的Southern印迹和蛋白质印迹检测16K rPRL的mRNA和蛋白质表达水平。当导入表达载体时，结果如下：第一，16K rPRL与rPRL结合可减少睾丸中的血管生成，而单独的rPRL则诱导血管生成。第二，16K rPRL与rPRL结合可减少白细胞增殖，而单独的rPRL则增加白细胞增殖。第三，16K rPRL与rPRL结合可缩短动情间期，而单独的rPRL则延长动情间期。第四，16K rPRL与rPRL结合意外地增加了睾酮（T）水平，而单独的rPRL则未增加T水平。综上所述，我们的数据表明，16K rPRL异构体在睾丸血管生成、白细胞增殖和生殖中发挥整体功能，尽管16K rPRL的作用并不总是拮抗的。
