• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

远端切缘 CD133 表达增强与结直肠癌预后不良相关。

Augmented CD133 expression in distal margin correlates with poor prognosis in colorectal cancer.

机构信息

Cancer Research Program 4, Rajiv Gandhi Centre for Biotechnology, Trivandrum, Kerala, India.

Department of surgical oncology, Regional Cancer Centre, Trivandrum, Kerala, India.

出版信息

J Cell Mol Med. 2019 Jun;23(6):3984-3994. doi: 10.1111/jcmm.14284. Epub 2019 Apr 4.

DOI:10.1111/jcmm.14284
PMID:30950180
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6533563/
Abstract

Pathological assessment of excised tumour and surgical margins in colorectal cancer (CRC) play crucial role in prognosis after surgery. Molecular assessment of margins could be more sensitive and informative than conventional histopathological analysis. Considering this view, we evaluated the distal surgical margins for expression of cancer stem cell (CSC) markers. Cellular and molecular assessment of normal, tumour and distal margin tissues were performed by flow cytometry, real-time q-PCR and immuno-histochemical analysis for CRC patients after tumour excision. CRC patients were evaluated for expression of CSC markers in their normal, tumour and distal tissues. Flow cytometry assay revealed CD133 and CD44 enriched cells in distal margin and tumour compared to normal colorectal tissues, which was further confirmed by immunohistochemistry. Most importantly, immunohistochemistry also revealed the enrichment of CSC markers expression in pathologically negative distal margins. Patients with distal margin enriched for CD133 expression showed an increased recurrence rate and decreased disease-free survival. This study proposes that although distal margin seems to be tumour free in conventional histopathological analysis, it could harbour cells enriched for CSC markers. Further CD133 could be a promising molecule to be used in molecular pathology for disease prognosis after surgery in CRC patients.

摘要

在结直肠癌（CRC）中，切除肿瘤和手术切缘的病理评估对手术后的预后起着至关重要的作用。与传统的组织病理学分析相比，对切缘的分子评估可能更敏感和更具信息量。基于这一观点，我们评估了远端手术切缘中癌症干细胞（CSC）标志物的表达情况。对接受肿瘤切除的 CRC 患者的正常、肿瘤和远端组织进行了流式细胞术、实时 q-PCR 和免疫组织化学分析，以评估肿瘤细胞的细胞和分子状态。评估了 CRC 患者正常、肿瘤和远端组织中 CSC 标志物的表达情况。流式细胞术检测显示，与正常结直肠组织相比，远端切缘和肿瘤组织中 CD133 和 CD44 阳性细胞明显增多，免疫组织化学进一步证实了这一点。更为重要的是，免疫组织化学还显示出 CSC 标志物在病理上阴性的远端切缘中的表达富集。CD133 表达丰富的远端切缘患者复发率增加，无病生存率降低。这项研究表明，尽管在常规组织病理学分析中远端切缘似乎没有肿瘤，但它可能含有富含 CSC 标志物的细胞。进一步的研究表明，CD133 可能是一种很有前途的分子，可用于 CRC 患者手术后的分子病理学中预测疾病预后。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d84/6533563/3d3916252f2d/JCMM-23-3984-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d84/6533563/3934ca255eb3/JCMM-23-3984-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d84/6533563/55b64fddb60a/JCMM-23-3984-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d84/6533563/bea7ad96c2f6/JCMM-23-3984-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d84/6533563/3d3916252f2d/JCMM-23-3984-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d84/6533563/3934ca255eb3/JCMM-23-3984-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d84/6533563/55b64fddb60a/JCMM-23-3984-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d84/6533563/bea7ad96c2f6/JCMM-23-3984-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d84/6533563/3d3916252f2d/JCMM-23-3984-g004.jpg

相似文献

1
Augmented CD133 expression in distal margin correlates with poor prognosis in colorectal cancer.远端切缘 CD133 表达增强与结直肠癌预后不良相关。
J Cell Mol Med. 2019 Jun;23(6):3984-3994. doi: 10.1111/jcmm.14284. Epub 2019 Apr 4.
2
Evaluation of CD44 and CD133 as cancer stem cell markers for colorectal cancer.评估 CD44 和 CD133 作为结直肠癌的肿瘤干细胞标志物。
Oncol Rep. 2012 Oct;28(4):1301-8. doi: 10.3892/or.2012.1951. Epub 2012 Aug 6.
3
Immunohistochemical detection of cancer stem cell related markers CD44 and CD133 in metastatic colorectal cancer patients.转移性结直肠癌患者中癌症干细胞相关标志物CD44和CD133的免疫组织化学检测
Biomed Res Int. 2014;2014:432139. doi: 10.1155/2014/432139. Epub 2014 Apr 22.
4
The Prognostic Value of Cancer Stem Cell Markers (Notch1, ALDH1, and CD44) in Primary Colorectal Carcinoma.癌症干细胞标志物（Notch1、醛脱氢酶1和CD44）在原发性结直肠癌中的预后价值
J Gastrointest Cancer. 2019 Dec;50(4):824-837. doi: 10.1007/s12029-018-0156-6.
5
LGR5 and CD133 as prognostic and predictive markers for fluoropyrimidine-based adjuvant chemotherapy in colorectal cancer.LGR5和CD133作为结直肠癌氟尿嘧啶类辅助化疗的预后和预测标志物。
Acta Oncol. 2016 Dec;55(12):1425-1433. doi: 10.1080/0284186X.2016.1201215. Epub 2016 Jul 20.
6
Microenvironmentally-driven Plasticity of CD44 isoform expression determines Engraftment and Stem-like Phenotype in CRC cell lines.微环境驱动的 CD44 异构体表达的可塑性决定了结直肠癌细胞系的植入和干性表型。
Theranostics. 2020 Jun 18;10(17):7599-7621. doi: 10.7150/thno.39893. eCollection 2020.
7
Putative cancer stem cells are present in surgical margins of oral squamous cell carcinoma.口腔鳞状细胞癌手术切缘中存在假定的癌症干细胞。
J BUON. 2018 Nov-Dec;23(6):1686-1692.
8
mTOR Signaling Combined with Cancer Stem Cell Markers as a Survival Predictor in Stage II Colorectal Cancer.mTOR 信号通路联合肿瘤干细胞标志物作为Ⅱ期结直肠癌的生存预测指标。
Yonsei Med J. 2020 Jul;61(7):572-578. doi: 10.3349/ymj.2020.61.7.572.
9
Characterization of cancer stem cells from different grades of human colorectal cancer.不同分级的人类结直肠癌中癌症干细胞的特征分析
Tumour Biol. 2016 Oct;37(10):14069-14081. doi: 10.1007/s13277-016-5232-6. Epub 2016 Aug 9.
10
Differential expression of CD133 based on microsatellite instability status in human colorectal cancer.基于微卫星不稳定性状态的 CD133 在人结直肠癌中的差异表达。
Mol Carcinog. 2014 Feb;53 Suppl 1:E1-10. doi: 10.1002/mc.21971. Epub 2012 Oct 12.

引用本文的文献

1
CD11b/CD86 involved in the microenvironment of colorectal cancer by promoting Wnt signaling activation.CD11b/CD86 通过促进 Wnt 信号激活参与结直肠癌的微环境。
Cancer Med. 2024 Sep;13(18):e70245. doi: 10.1002/cam4.70245.
2
GART Functions as a Novel Methyltransferase in the RUVBL1/β-Catenin Signaling Pathway to Promote Tumor Stemness in Colorectal Cancer.GART 在 RUVBL1/β-连环蛋白信号通路中作为一种新型甲基转移酶促进结直肠癌的肿瘤干性。
Adv Sci (Weinh). 2023 Sep;10(25):e2301264. doi: 10.1002/advs.202301264. Epub 2023 Jul 13.
3
Pleiotropic effects of DCLK1 in cancer and cancer stem cells.

本文引用的文献

1
COSMIC: the Catalogue Of Somatic Mutations In Cancer.COSMIC：癌症体细胞突变目录。
Nucleic Acids Res. 2019 Jan 8;47(D1):D941-D947. doi: 10.1093/nar/gky1015.
2
Colorectal Cancer in India: An Audit from a Tertiary Center in a Low Prevalence Area.印度的结直肠癌：来自低发病率地区一家三级中心的审计
Indian J Surg Oncol. 2017 Dec;8(4):484-490. doi: 10.1007/s13193-017-0655-0. Epub 2017 Apr 22.
3
Risk Factors Predicting Colorectal Cancer Recurrence Following Initial Treatment: A 5-year Cohort Study.初始治疗后预测结直肠癌复发的危险因素：一项5年队列研究。
DCLK1在癌症和癌症干细胞中的多效性作用。
Front Mol Biosci. 2022 Sep 26;9:965730. doi: 10.3389/fmolb.2022.965730. eCollection 2022.
4
Evaluation of CD133 and CD56/NCAM expression in Wilms tumor and their association with prognostic factors.肾母细胞瘤中CD133和CD56/神经细胞黏附分子表达的评估及其与预后因素的关联。
Iran J Basic Med Sci. 2020 Jul;23(7):853-857. doi: 10.22038/ijbms.2020.41468.9804.
5
Clinical significance of CD133 and Nestin in astrocytic tumor: The correlation with pathological grade and survival.CD133 和 Nestin 在星形细胞瘤中的临床意义：与病理分级和生存的相关性。
J Clin Lab Anal. 2020 Mar;34(3):e23082. doi: 10.1002/jcla.23082. Epub 2019 Nov 1.
Asian Pac J Cancer Prev. 2017 Sep 27;18(9):2465-2470. doi: 10.22034/APJCP.2017.18.9.2465.
4
Cancer stem cells: The root of tumor recurrence and metastases.肿瘤干细胞：肿瘤复发和转移的根源。
Semin Cancer Biol. 2017 Jun;44:10-24. doi: 10.1016/j.semcancer.2017.02.011. Epub 2017 Feb 28.
5
CD133+CD24lo defines a 5-Fluorouracil-resistant colon cancer stem cell-like phenotype.CD133+CD24lo定义了一种对5-氟尿嘧啶耐药的结肠癌干细胞样表型。
Oncotarget. 2016 Nov 29;7(48):78698-78712. doi: 10.18632/oncotarget.12168.
6
Importance of surgical margins in rectal cancer.手术切缘在直肠癌中的重要性。
J Surg Oncol. 2016 Mar;113(3):323-32. doi: 10.1002/jso.24136.
7
The role of TWIST1 in epithelial-mesenchymal transition and cancers.TWIST1在上皮-间质转化及癌症中的作用。
Tumour Biol. 2016 Jan;37(1):185-97. doi: 10.1007/s13277-015-4450-7. Epub 2015 Nov 24.
8
Clinical influence of cancer stem cells on residual disease after preoperative chemoradiotherapy for rectal cancer.癌症干细胞对直肠癌术前放化疗后残留疾病的临床影响。
Tumour Biol. 2016 Mar;37(3):3571-80. doi: 10.1007/s13277-015-4201-9. Epub 2015 Oct 11.
9
Molecular margin of surgical resections--where do we go from here?手术切除的分子边界——我们从这里走向何方？
Cancer. 2015 Jun 15;121(12):1914-6. doi: 10.1002/cncr.29299. Epub 2015 Mar 13.
10
CD133-positive tumor cell content is a predictor of early recurrence in colorectal cancer.CD133 阳性肿瘤细胞含量是结直肠癌早期复发的预测指标。
J Gastrointest Oncol. 2014 Dec;5(6):447-56. doi: 10.3978/j.issn.2078-6891.2014.071.