Department of Neurochemistry, Institute of Psychiatry and Neurology, Sobieskiego Street 9, 02-957 Warsaw, Poland.
Department of Neurochemistry, Institute of Psychiatry and Neurology, Sobieskiego Street 9, 02-957 Warsaw, Poland; Department of Experimental and Clinical Pharmacology, Centre for Preclinical Research and Technology CePT, Medical University of Warsaw, Banacha 1B, 02-097 Warsaw, Poland.
J Neuroimmunol. 2019 Jul 15;332:57-63. doi: 10.1016/j.jneuroim.2019.03.018. Epub 2019 Mar 29.
In our study, we assessed the potency of the brain-derived proteins ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), matrix metalloproteinase 9 (MMP-9), glial fibrillary acidic protein (GFAP) and the immune activation indicators interleukin 1β (IL-1β) and interleukin 6 (IL-6) as peripheral biomarkers of different susceptibilities to kindling in a preclinical model. We observed increased plasma UCH-L1 levels in kindled vs. control animals. Furthermore, MMP-9 and IL-1β concentrations were the lowest in rats resistant to kindling. In summary, UCH-L1 is an indicator of neuronal loss and BBB disruption after seizure. MMP-9 and IL-1β may indicate resistance to kindling. UCH-L1, MMP-9 and IL-1β may have utility as peripheral biomarkers with translational potency in the clinic.
在我们的研究中，我们评估了脑源性蛋白泛素羧基末端水解酶 L1（UCH-L1）、基质金属蛋白酶 9（MMP-9）、神经胶质纤维酸性蛋白（GFAP）以及免疫激活指标白细胞介素 1β（IL-1β）和白细胞介素 6（IL-6）作为临床前模型中不同易化易感性的外周生物标志物的效力。我们观察到在点燃的动物中血浆 UCH-L1 水平升高。此外，在对点燃有抵抗力的大鼠中，MMP-9 和 IL-1β 的浓度最低。总之，UCH-L1 是癫痫发作后神经元丢失和 BBB 破坏的指标。MMP-9 和 IL-1β 可能表明对易化的抵抗力。UCH-L1、MMP-9 和 IL-1β 可用作具有临床转化潜力的外周生物标志物。
