基于适配体/NH 介孔双球粒子的无标记适体传感器用于赭曲霉毒素 A 的超灵敏电化学检测。
A Label-free aptasensor based on Aptamer/NH Janus particles for ultrasensitive electrochemical detection of Ochratoxin A.
机构信息
School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China.
Institute of Environmental Science, Shanxi University, Taiyuan 030006, China.
出版信息
Talanta. 2019 Jul 1;199:310-316. doi: 10.1016/j.talanta.2019.02.015. Epub 2019 Feb 11.
In this study, a novel aptasensor based on Aptamer/NH Janus particles is developed for the detection of Ochratoxin A(OTA). By coating gold on the hemispherical surface of the aminated polystyrene particles, Ochratoxin A aptamer is immobilized on the surface of the gold layer for selective identification and the other hemispherical able to bind to Glassy carbon electrode via peptide bond. Under optimum conditions, the sensor exhibited a wide dynamic range of OTA concentration from 1 × 10 nM to 10 nM, and the detection limit is 3.3 × 10 pM on condition of acceptable stability and reproducibility. The sensors were showed excellent performance in the detection of OTA in red wine sample with recoveries between 95.7% and 100.18%, which studied by the standard addition spiking technique. This work provides a new idea and method for preparing immune electrochemical sensors and is expected to be used for the OTA detection in red wine sample analysis.
本研究开发了一种基于适体/ NH 双节粒子的新型适体传感器，用于检测赭曲霉毒素 A（OTA）。通过在氨基化聚苯乙烯颗粒的半球形表面上镀上金，将 OTA 适体固定在金层表面上，以进行选择性识别，而另一个半球形则通过肽键结合到玻碳电极上。在最佳条件下，该传感器在 OTA 浓度为 1×10 nM 至 10 nM 的范围内表现出宽的动态范围，在可接受的稳定性和重现性条件下，检测限为 3.3×10 pM。该传感器在红酒样品中的 OTA 检测中表现出优异的性能，回收率在 95.7%至 100.18%之间，这是通过标准添加加标技术研究的。这项工作为制备免疫电化学传感器提供了新的思路和方法，有望用于红酒样品中 OTA 的检测。
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