State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China; National Engineering Laboratory for Sustainable Sludge Management & Resourcelization Technology, Harbin Institute of Technology, Harbin 150090, China.
J Environ Sci (China). 2019 Jun;80:229-239. doi: 10.1016/j.jes.2018.12.016. Epub 2018 Dec 31.
The effects of silver nanoparticles (AgNPs) on reactor performance, extracellular polymeric substances composition and microbial community structure and function in integrated fixed-film activated sludge-sequencing batch reactors (IFAS-SBRs) were investigated. Results showed that the addition of AgNPs from 0.1 to 10 mg/L exhibited no significant effects on nutrient removal. The average overall removal of COD, NH-N and PO-P was 96.6%, 99.9% and 98.8%, respectively. The introduction of AgNPs caused an increase in extracellular polymeric substances content for the sludge and biofilm of IFAS-SBRs. The release of Ag from AgNPs and lactate dehydrogenase test implied the low toxicity of AgNPs to IFAS-SBRs. High-throughput sequencing revealed that microbial community structure showed significant shifts at phylum and genus levels after long-term exposure to AgNPs, but core functional groups responsible for nutrient removal remained at high abundance. Bacterial function prediction indicated that the metabolic categories showed no significant shifts under AgNPs stress, therefore good process performance could still be achieved.
研究了银纳米粒子（AgNPs）对一体式固定膜活性污泥-序批式反应器（IFAS-SBR）中反应器性能、胞外聚合物组成以及微生物群落结构和功能的影响。结果表明，添加 0.1-10mg/L 的 AgNPs 对养分去除没有显著影响。COD、NH-N 和 PO-P 的平均总去除率分别为 96.6%、99.9%和 98.8%。AgNPs 的引入导致 IFAS-SBR 中污泥和生物膜的胞外聚合物含量增加。AgNPs 中的 Ag 释放和乳酸脱氢酶测试表明 AgNPs 对 IFAS-SBR 的毒性较低。高通量测序显示，微生物群落结构在长期暴露于 AgNPs 后在门和属水平上发生了显著变化，但负责去除养分的核心功能群仍然保持高丰度。细菌功能预测表明，在 AgNPs 胁迫下代谢类别没有明显变化，因此仍能实现良好的工艺性能。
