Key Laboratory for Regenerative Medicine of Ministry of Education, Institute of Hematology, School of Medicine, Jinan University, Guangzhou, China.
Department of Hematology, Guangzhou First Municipal People's Hospital, The Second Affiliated Hospital of South China University of Technology, Guangzhou, China.
Blood Cells Mol Dis. 2019 Jul;77:34-42. doi: 10.1016/j.bcmd.2019.03.009. Epub 2019 Mar 30.
Aplastic anemia (AA) is T cell immune-mediated autoimmune disease. Aberrant T cell activation involves an imbalance in T cell homeostasis in AA. However, whether the T cell activation molecule CD27 and its ligand CD70 participate in the immune pathogenesis of AA remains ill defined.
The frequencies of CD27/CD70 and perforin/granzyme B in different T cell subsets were detected in AA patients and healthy individuals by flow cytometry.
We first time demonstrate a significantly elevated proportion of CD27 and significantly decreased CD70 T cells from AA. Changed frequency of CD27 and CD70 in different T cell subsets appeared to be associated with AA severity. In very severe aplastic anemia (VSAA) and severe aplastic anemia (SAA), increased CD8CD27 T cells present with a cytotoxic effector phenotype by elevating perforin proportion.
Elevated proportion of CD27 in T cells may contribute to distinct immune pathogenesis for different severities of AA. The CD8CD27perforin T cells combined with CD8CD70 T cells may serve as an immune biomarker for AA severity estimation.
再生障碍性贫血（AA）是 T 细胞免疫介导的自身免疫性疾病。异常的 T 细胞激活涉及 AA 中 T 细胞稳态失衡。然而，T 细胞活化分子 CD27 及其配体 CD70 是否参与 AA 的免疫发病机制仍不清楚。
通过流式细胞术检测 AA 患者和健康个体不同 T 细胞亚群中 CD27/CD70 和穿孔素/颗粒酶 B 的频率。
我们首次证明 AA 患者 CD27 和 CD70 T 细胞的比例显著升高。不同 T 细胞亚群中 CD27 和 CD70 的频率变化似乎与 AA 的严重程度有关。在极重型再生障碍性贫血（VSAA）和重型再生障碍性贫血（SAA）中，升高的 CD8CD27 T 细胞通过提高穿孔素比例呈现出细胞毒性效应表型。
T 细胞中 CD27 比例的升高可能导致不同严重程度 AA 的独特免疫发病机制。CD8CD27perforin T 细胞与 CD8CD70 T 细胞相结合可能作为 AA 严重程度估计的免疫生物标志物。
