通过测定 Nrf1 和抗氧化系统相关基因评估对大型溞的对乙酰氨基酚的氧化应激。
Assessment of oxidative stress of paracetamol to Daphnia magna via determination of Nrf1 and genes related to antioxidant system.
机构信息
Department of Ecology, Jinan University, Guangzhou, 510632, China.
Department of Ecology, Jinan University, Guangzhou, 510632, China; Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Jinan University, Guangzhou, 510632, China.
出版信息
Aquat Toxicol. 2019 Jun;211:73-80. doi: 10.1016/j.aquatox.2019.03.014. Epub 2019 Mar 28.
Paracetamol (APAP) is one of the most widely used anti-inflammatory and analgesic drugs in human being health care and has been universally detected in various aquatic environments. However, its potential adverse effects and toxic mechanisms on freshwater invertebrates still remain unclear. In the present study, the effects of APAP on the expressions of Nrf1 and the antioxidant related genes including GCLC, GST, GPX, CAT, TRX, TrxR and Prx1 in Daphnia magna (D. magna) were evaluated after 24, 48 and 96 h, and the changes of GPX, GST and CAT enzyme activities, as well as the GSH and MDA content under APAP exposure for 48 h were also determined. Results showed that paracetamol affected the expressions of Nrf1 and antioxidant related genes in D. magna, which were related to the exposure time and concentration of APAP. Nrf1 was inhibited at 48 h, but induced at 96 h under the APAP exposure, being about two fold of the control in 5.0 μg/L. CAT were significantly induced in all treatments. But Prx decreased in an concentration-dependent manner in all treatments. In comparison with the mRNA expression, antioxidant enzymes activity displayed less changes in D. magna. Overall, APAP exposure altered the expression of Nrf1 and genes related to antioxidant system and disturbed the redox homeostasis of D. magna.
对乙酰氨基酚（APAP）是人类保健中应用最广泛的抗炎和镇痛药之一，已在各种水生环境中普遍检出。然而，其对淡水无脊椎动物的潜在不良影响和毒性机制仍不清楚。在本研究中，在 24、48 和 96 h 后，评估了 APAP 对大型溞（Daphnia magna）中 Nrf1 及其抗氧化相关基因（包括 GCLC、GST、GPX、CAT、TRX、TrxR 和 Prx1）表达的影响，并在 APAP 暴露 48 h 时测定了 GPX、GST 和 CAT 酶活性以及 GSH 和 MDA 含量的变化。结果表明，对乙酰氨基酚影响了 D. magna 中 Nrf1 和抗氧化相关基因的表达，这与 APAP 的暴露时间和浓度有关。APAP 暴露 48 h 时 Nrf1 受到抑制，但 96 h 时被诱导，在 5.0μg/L 时约为对照的两倍。在所有处理中，CAT 均显著诱导。但 Prx 在所有处理中均呈浓度依赖性下降。与 mRNA 表达相比，抗氧化酶活性在 D. magna 中变化较小。总体而言，APAP 暴露改变了 Nrf1 的表达和抗氧化系统相关基因，并扰乱了 D. magna 的氧化还原稳态。
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