Heo Hee Sun, Han Ga Eun, Won Junho, Cho Yeonoh, Woo Hyeran, Lee Jong Hun
Department of Food Science and Biotechnology, College of Life Sciences, CHA University, Gyeonggi-do 11160, Republic of Korea.
J Microbiol Biotechnol. 2019 Apr 28;29(4):518-526. doi: 10.4014/jmb.1812.12019.
var. is a bioactive substance, in possession of a variety of beneficial health effects, which has long been extensively used as a traditional medication for the treatment of fever, acute dysentery, diabetes, and cardiovascular diseases in North-East Asian countries. The purpose of this study was to evaluate the cytoprotective activity of var. lobata ethanol extract (PLE) for ultraviolet B (UVB) induced oxidative stress in human dermal fibroblasts (HDF). It was hypothesized that PLE treatment (25-100 μg/mL) would reduce intracellular reactive oxygen species (ROS) levels as well as increase collagen production in UVB-irradiated HDF. The results confirmed this theory, with collagen production increasing in the PLE treatment group in a dose-dependent manner. In addition, regulators of cellular ROS accumulation, including HO-1 and NOQ-1, were activated by Nrf2, which was mediated by PLE. Hence, intracellular levels of ROS were also reduced in the PLE treatment group in a dose-dependent manner. In conclusion, PLE increases collagen production and maintains hyaluronic acid (HA) levels in human dermal fibroblasts exposed to UVB-irradiation, thereby inhibiting photoaging.
变种是一种生物活性物质，具有多种有益的健康功效，长期以来在东北亚国家被广泛用作治疗发热、急性痢疾、糖尿病和心血管疾病的传统药物。本研究的目的是评估变种乙醇提取物（PLE）对人皮肤成纤维细胞（HDF）中紫外线B（UVB）诱导的氧化应激的细胞保护活性。研究假设PLE处理（25 - 100μg/mL）会降低细胞内活性氧（ROS）水平，并增加UVB照射的HDF中的胶原蛋白生成。结果证实了这一理论，PLE处理组中的胶原蛋白生成呈剂量依赖性增加。此外，包括HO - 1和NOQ - 1在内的细胞ROS积累调节剂被Nrf2激活，这是由PLE介导的。因此，PLE处理组中的细胞内ROS水平也呈剂量依赖性降低。总之，PLE增加了暴露于UVB照射的人皮肤成纤维细胞中的胶原蛋白生成并维持透明质酸（HA）水平，从而抑制光老化。
