Yamazaki S, Shimazu T, Kimura S, Shimizu H
J Interferon Res. 1986 Aug;6(4):331-6. doi: 10.1089/jir.1986.6.331.
The complete amino acid sequence of recombinant human gamma-interferon (HuIFN-gamma) produced in Escherichia coli was determined using a gas-phase protein sequencer. The sequence was established by automated Edman degradation on the intact protein and its peptides obtained after Staphylococcus aureus V8 protease or trypsin digestion. The result was identical to the amino acid sequence predicted from the nucleotide sequence of the cloned HuIFN-gamma cDNA except that it was missing the four carboxy-terminal residues, Arg-Ala-Ser-Gln.
利用气相蛋白质测序仪测定了在大肠杆菌中产生的重组人γ干扰素（HuIFN-γ）的完整氨基酸序列。该序列是通过对完整蛋白质及其在金黄色葡萄球菌V8蛋白酶或胰蛋白酶消化后获得的肽段进行自动Edman降解来确定的。结果与从克隆的HuIFN-γ cDNA核苷酸序列预测的氨基酸序列相同，只是缺少四个羧基末端残基，即精氨酸-丙氨酸-丝氨酸-谷氨酰胺。
