Gourley G R, Arend R, Mogilevsky W S, Odell G B, Siegel F L
J Lab Clin Med. 1986 Nov;108(5):436-41.
High-performance liquid chromatography was used to analyze the composition of bilirubin conjugates in bile from adult male and female outbred normal (JJ) and heterozygous (Jj) Gunn rats. Bile was examined directly without prior derivatization or extraction. Significantly higher bilirubin diglucuronide and lower bilirubin monoglucuronide (both C-8 and C-12 isomers) excretion in JJ rats was demonstrated. Bilirubin monoglucuronide C-8/C-12 ratios were similar in both genotypes, as was the percentage of bilirubin monoglucuronide monoglycoside diester. Hepatic bilirubin uridine diphosphoglucuronyltransferase activity showed a statistically significant positive correlation with the relative amount of bilirubin diglucuronide present in bile and a significant negative correlation with the amount of bilirubin monoglucuronide. The relative percentage of bilirubin monoglucuronide vs. diglucuronide in bile allows an indirect assessment of hepatic bilirubin uridine diphosphoglucuronyltransferase activity.
采用高效液相色谱法分析成年雄性和雌性远交系正常（JJ）和杂合子（Jj）冈恩大鼠胆汁中胆红素结合物的组成。胆汁未经预先衍生化或提取直接进行检测。结果表明，JJ大鼠中胆红素二葡萄糖醛酸酯排泄显著更高，而胆红素单葡萄糖醛酸酯（C - 8和C - 12异构体）排泄更低。两种基因型的胆红素单葡萄糖醛酸酯C - 8/C - 12比值相似，胆红素单葡萄糖醛酸酯单糖苷二酯的百分比也相似。肝脏胆红素尿苷二磷酸葡萄糖醛酸基转移酶活性与胆汁中胆红素二葡萄糖醛酸酯的相对含量呈统计学显著正相关，与胆红素单葡萄糖醛酸酯的含量呈显著负相关。胆汁中胆红素单葡萄糖醛酸酯与二葡萄糖醛酸酯的相对百分比可间接评估肝脏胆红素尿苷二磷酸葡萄糖醛酸基转移酶活性。
