Key Laboratory of Optic-Electric Sensing and Analytical Chemistry for Life Science, MOE, Key Laboratory of Analytical Chemistry for Life Science in Universities of Shandong, Shandong Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, PR China.
Key Laboratory of Optic-Electric Sensing and Analytical Chemistry for Life Science, MOE, Key Laboratory of Analytical Chemistry for Life Science in Universities of Shandong, Shandong Key Laboratory of Biochemical Analysis, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, PR China.
Biosens Bioelectron. 2019 Jun 1;134:42-48. doi: 10.1016/j.bios.2019.03.048. Epub 2019 Mar 28.
A simple "signal-on" photoelectrochemical (PEC) aptasensor is constructed for Aflatoxin B1 (AFB1) detection based on electrochemically reduced graphene oxide/poly(5-formylindole)/Au (erGO/P5FIn/Au) nanocomposites. The nanocomposites are synthesized by simple electrochemical deposition method and show good photoelectrochemical performance. Poly(5-formylindole) (P5FIn) can generate electron-hole pairs under light irradiation, leading to the formation of robust cathode photocurrent. Au can be acted as signal amplifier due to the high conductivity. The erGO is used to immobilize AFB1 aptamer chain by π-π stacking interaction between the carbon six-membered ring in graphene and the C-N heterocyclic ring in nucleobases of ssDNA. After the insulating AFB1 aptamer chain is fixed to the electrode, the signal of PEC sensor is "OFF". In the process of AFB1 detection, the aptamer chain detaches from the surface of erGO, which results in "ON" of the sensor signal. Based on this design, this constructed PEC aptasensor shows a high sensitivity for AFB1 with a wide linear detection range (LDR) from 0.01 ng mL to 100 ng mL. The limit of detection (LOD) is 0.002 ng mL. This PEC sensor also exhibits good stability, selectivity, specificity, and satisfactory practical sample analysis ability. This work may provide a new promising PEC platform for AFB1 detection as well as some other small molecules analysis.
基于电化学还原氧化石墨烯/聚（5-甲酰基吲哚）/Au（erGO/P5FIn/Au）纳米复合材料构建了一种简单的“信号开启”光电化学（PEC）适体传感器，用于检测黄曲霉毒素 B1（AFB1）。该纳米复合材料通过简单的电化学沉积方法合成，表现出良好的光电化学性能。聚（5-甲酰基吲哚）（P5FIn）在光照下能产生电子-空穴对，从而形成强的阴极光电流。Au 由于其高导电性可作为信号放大器。erGO 用于通过石墨烯中环的碳六元环与 ssDNA 中碱基的 C-N 杂环之间的π-π堆积相互作用固定 AFB1 适体链。在绝缘的 AFB1 适体链固定到电极后，PEC 传感器的信号为“关闭”。在 AFB1 检测过程中，适体链从 erGO 表面脱离，从而导致传感器信号“开启”。基于此设计，所构建的 PEC 适体传感器对 AFB1 表现出高灵敏度，具有从 0.01ng/mL 到 100ng/mL 的宽线性检测范围（LDR）。检测限（LOD）为 0.002ng/mL。该 PEC 传感器还表现出良好的稳定性、选择性、特异性和令人满意的实际样品分析能力。这项工作可能为 AFB1 检测以及其他一些小分子分析提供了一种有前途的 PEC 平台。
