亚冰点温度下等容系统中大鼠心脏保存期间压力的时间依赖性效应
Time-dependent Effects of Pressure during Preservation of Rat Hearts in an Isochoric System at Subfreezing Temperatures.
作者信息
Wan L, Powell-Palm M J, Clemens M G, Rubinsky B
机构信息
Department of Cardiovascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; Department of Mechanical Engineering & Department of Bioengineering, University of California, Berkeley, USA.
Department of Mechanical Engineering & Department of Bioengineering, University of California, Berkeley, USA.
出版信息
Cryo Letters. 2019 Jan/Feb;40(1):64-70.
BACKGROUND
Isochoric freezing systems enable ice-free preservation of biological matter at subfreezing temperatures under the increased hydrostatic pressure.
OBJECTIVE
To examine the effects of pressure and exposure period on rat hearts preserved in an isochoric chamber.
MATERIALS AND METHODS
Rat hearts were preserved in the UW solution in isochoric chambers at temperatures from -2°C to -8°C and pressure from the atmospheric level to 78 MPa for up to eight hours, with and without the addition of glycerol. Hearts were evaluated via Langendorff perfusion and HE histology.
RESULTS
Hearts were compromised quickly as pressure increased, suggesting an acute time-pressure sensitivity. With the addition of 1 M glycerol, which reduces the pressure experienced at a given temperature, the survival time at -4°C was doubled.
CONCLUSION
The enhanced hydrostatic pressure encountered during isochoric preservation yields time-dependent negative effects on the heart, which can potentially be alleviated by the addition of a cryoprotectant.
背景
等容冷冻系统能够在亚冰点温度下、增加的静水压力条件下对生物物质进行无冰保存。
目的
研究压力和暴露时间对保存在等容腔室中的大鼠心脏的影响。
材料与方法
将大鼠心脏置于UW溶液中，在等容腔室中于-2°C至-8°C的温度以及从大气压至78 MPa的压力下保存长达8小时，添加和不添加甘油。通过Langendorff灌注和HE组织学对心脏进行评估。
结果
随着压力增加，心脏功能迅速受损，表明存在急性时间-压力敏感性。添加1 M甘油可降低给定温度下所承受的压力，在-4°C时存活时间延长了一倍。
结论
等容保存过程中遇到的增强静水压力对心脏产生时间依赖性负面影响，添加冷冻保护剂可能会缓解这种影响。
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