Veterinary Bacteriology and Pathology, Research and Laboratory Division, Finnish Food Authority, Neulaniementie 4, 70210 Kuopio, Finland.
Veterinary Bacteriology and Pathology, Research and Laboratory Division, Finnish Food Authority, Neulaniementie 4, 70210 Kuopio, Finland.
Vet Microbiol. 2019 Apr;231:107-115. doi: 10.1016/j.vetmic.2019.03.007. Epub 2019 Mar 9.
Mycoplasma bovis causes bovine respiratory disease, mastitis, arthritis and otitis. The importance of M. bovis has escalated because of recent outbreaks and introductions into countries previously free of M. bovis. We characterized the course of M. bovis infection on 19 recently infected dairy farms over 24 months. Our objective was to identify diagnostic tools to assess the efficacy of control measures to assess low risk infection status on M. bovis infected farms. PCR assays and culture were used to detect M. bovis, and in-house and BioX ELISAs were used to follow antibody responses. Cows and young stock were sampled on four separate occasions, and clinical cases were sampled when they arose. On 17 farms, a few cases of clinical mastitis were detected, mostly within the first eight weeks after the index case. Antibodies detected by in-house ELISA persisted in the serum of cows at least for 1.5 years on all farms, regardless of the M. bovis infection status or signs of clinical disease or subclinical mastitis on the farm. Six out of 19 farms became low risk as the infection was resolved. Our results suggest that, for biosecurity purposes, regular monitoring should be conducted on herds by screening for M. bovis in samples from cows with clinical mastitis and calves with pneumonia, in conjunction with testing young stock by screening longitudinally collected nasal swabs for M. bovis and sequential serum samples for antibody against recombinant antigen.
牛支原体引起牛呼吸道疾病、乳腺炎、关节炎和中耳炎。由于最近的暴发和向以前无牛支原体的国家引入，牛支原体的重要性不断增加。我们在 24 个月内对 19 个最近感染的奶牛场的牛支原体感染过程进行了特征描述。我们的目的是确定诊断工具，以评估控制措施的效果，评估牛支原体感染场的低风险感染状态。PCR 检测和培养用于检测牛支原体，使用内部和 BioX ELISA 来跟踪抗体反应。在四个不同的时间点对奶牛和小牛进行采样，并在出现临床病例时进行采样。在 17 个农场中，检测到少数几例临床乳腺炎，主要发生在指数病例后的前 8 周内。在所有农场中，无论牛支原体感染状态如何，无论农场是否有临床疾病或亚临床乳腺炎的迹象，内部 ELISA 检测到的抗体至少在血清中持续存在 1.5 年。19 个农场中有 6 个农场由于感染得到解决而成为低风险。我们的研究结果表明，为了生物安全目的，应通过对有临床乳腺炎的奶牛和有肺炎的小牛的样本中牛支原体的筛选，以及对牛支原体和针对重组抗原的抗体的连续血清样本进行纵向采集，对牛群进行常规监测。
