ANSES, Hygiene and Quality of Poultry and Pig Products Unit, BP 53, 22440 Ploufragan, France; Faculty of Veterinary Medicine and Zootechnics, Central University of Ecuador, Quito, Ecuador.
ANSES, Hygiene and Quality of Poultry and Pig Products Unit, BP 53, 22440 Ploufragan, France.
Vet Microbiol. 2019 Apr;231:147-153. doi: 10.1016/j.vetmic.2019.03.003. Epub 2019 Mar 6.
Salmonella serovars Derby, Typhimurium and the monophasic variant of Salmonella Typhimurium are the most frequently isolated serovars in pigs in France. To compare the excretion patterns, seroconversion to Salmonella and contamination of the organs of pigs inoculated with strains of all three serovars, we conducted an experimental trial with 28 SPF piglets. Four were used as a negative control, while the other 24 were divided equally into three groups. Each group was inoculated at 7 weeks of age with a different strain: S. Derby (SDb), S. Typhimurium (ST), and the monophasic variant of S. Typhimurium (mST). Fecal and blood samples were collected twice a week up until necropsy, on 21 days post-inoculation (DPI) for half of each group and 49 DPI for the remaining piglets. During necropsy, the tonsils, mesenteric lymph nodes and various intestinal contents were collected from each pig. Salmonella bacteria were quantified in CFU/g by a bacteriological method, and levels of Salmonella antibodies were measured using an ELISA Kit. Piglets inoculated with mST continuously excreted Salmonella in their feces throughout the trial. For each of the other serovars, one piglet was Salmonella-negative on one DPI. The quantity of Salmonella excreted was statistically different between the group inoculated with ST and mST (p < 0.05), but no differences were found between the other serovars. The tonsils, cecum and jejunum were the most contaminated organs in all groups. Seroconversion for all the piglets was completed by different DPI: 28 for ST, 31 for mST and 38 for SDb. No major differences were found in terms of excretion and colonization among the studied serovars.
在法国，猪中最常分离到的血清型为德比沙门氏菌、肠炎沙门氏菌和肠炎沙门氏菌单相变异型。为了比较这三种血清型菌株接种猪的排泄模式、血清转换和器官污染情况，我们用 28 头 SPF 仔猪进行了一项实验。其中 4 头作为阴性对照，其余 24 头仔猪等分为三组。每组于 7 周龄时接种不同的菌株：德比沙门氏菌（SDb）、肠炎沙门氏菌（ST）和肠炎沙门氏菌单相变异型（mST）。每组的一半猪在接种后第 21 天（半数）和第 49 天（另一半）采集粪便和血液样本，直到剖检。剖检时，从每头猪采集扁桃体、肠系膜淋巴结和各种肠内容物。用细菌学方法以 CFU/g 定量检测沙门氏菌，用 ELISA 试剂盒检测沙门氏菌抗体水平。接种 mST 的仔猪在整个试验期间持续从粪便中排出沙门氏菌。对于其他两种血清型，有一头仔猪在一个 DPI 时为沙门氏菌阴性。接种 ST 和 mST 的仔猪的粪便中排出的沙门氏菌数量存在统计学差异（p<0.05），但其他血清型之间无差异。扁桃体、盲肠和空肠是所有组中污染最严重的器官。所有仔猪的血清转换都在不同的 DPI 完成：ST 为 28，mST 为 31，SDb 为 38。在研究的血清型中，排泄和定植没有明显差异。
