Ruiz-Terán Francisco, Martínez-Zepeda Paulina N, Geyer-de la Merced Sara Y, Nolasco-Cancino Hipócrates, Santiago-Urbina Jorge A
1Departamento de Alimentos y Biotecnología, Facultad de Química, Universidad Nacional Autónoma de México, 04510 Mexico City, Mexico.
2Facultad de Ciencias Químicas, Universidad Autónoma Benito Juárez de Oaxaca, 68120 Oaxaca de Juárez, Oaxaca Mexico.
Food Sci Biotechnol. 2018 Oct 16;28(2):459-467. doi: 10.1007/s10068-018-0490-2. eCollection 2019 Apr.
In this study, 19 indigenous mezcal strains were screened for their tolerance to grow under different stress conditions and their potential use in fermentation. All strains were able to tolerate pH value of 3, significant levels of glucose (30%), ethanol (12% v/v), and temperature of 37 °C. Eleven of them were able to grow in presence of 15% of ethanol, but only CH7 and PA18 strains grew at 42 °C. Both were selected for evaluation of their fermentative abilities in maguey juice and in a synthetic medium incubated at 30 and 40 °C. Temperature of 40 °C had a positive effect on the ethanol production, increasing the productivity and efficiency in maguey fermentation. Ethyl acetate, isobutanol and isoamyl alcohols production was favored at 30 °C. Both evaluated strains presented a good fermentative capacity and production of volatile compounds, suggesting their potential use as starter cultures in mezcal fermentation.
在本研究中，对19株本地龙舌兰酒菌株进行了筛选，以评估它们在不同应激条件下的生长耐受性及其在发酵中的潜在用途。所有菌株都能耐受pH值为3、高浓度葡萄糖（30%）、乙醇（12% v/v）以及37°C的温度。其中11株能够在15%乙醇存在的情况下生长，但只有CH7和PA18菌株能在42°C下生长。这两株菌株被选来评估它们在龙舌兰汁和在30°C和40°C下培养的合成培养基中的发酵能力。40°C的温度对乙醇产量有积极影响，提高了龙舌兰发酵的生产率和效率。在30°C时，乙酸乙酯、异丁醇和异戊醇的产量较高。所评估的两株菌株均表现出良好的发酵能力和挥发性化合物产量，表明它们有潜力用作龙舌兰酒发酵的起始培养物。
