Selek S, Esrefoglu M, Meral I, Bulut H, Caglar H G, Sonuc G, Yildiz C, Teloglu E S, Dogan N, Yuce B, Tiftik E, Bayindir N
a Departments of Medical Biochemistry , Bezmialem Vakif University , Istanbul , Turkey.
b Histology and Embryology , Bezmialem Vakif University , Istanbul , Turkey.
Biotech Histochem. 2019 Feb;94(2):75-83. doi: 10.1080/10520295.2018.1482001. Epub 2019 Apr 8.
We investigated the effects of Oenothera biennis L. and Hypericum perforatum L. extracts on brain tissue histopathology, myelin oligodendrocyte glycoprotein (MOG), myelin basic protein (MBP), total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) in mice with experimental autoimmune encephalomyelitis (EAE). Forty-seven C57BL/6J mice were divided into the following groups: multiple sclerosis (MS), control (healthy mice), MS + H. perforatum treated (MS + HP), MS + O. biennis treated (MS + OB). All groups except the control group were immunized by EAE methods. Two weeks after the immunization, the mice in the MS + HP group were fed normal food containing 18 - 21 g/kg H. perforatum extract, the mice in MS + OB group were fed normal food containing 18 - 21 g/kg O. biennis extract, and the mice in control and MS groups were fed normal food for six weeks. Brain tissue samples were collected from all mice for histopathological and biochemical analysis. Clinical signs of the disease were scored using functional systems scores (FSS) daily. The H. perforatum and O. biennis extracts ameliorated the increased brain tissue MOG and MBP values for animals with MS. H. perforatum and O. biennis extract decreased the TOS and OSI values for brain tissue and increased TAS levels in brain tissue of animals with MS. In addition, H. perforatum and O. biennis extracts decreased the clinical signs at the end of the experiment compared to the beginning of extract administration. We found that myelin was lost in MS group vs. control group. H. perforatum and O. biennis extract treatments decreased the amount of myelin loss in the MS + HP and MS + OB groups. We also observed amyloid deposition on vascular walls, in the cytoplasm of the neurons and in the intercellular space in the MS group. O. biennis and H. perforatum treated groups exhibited neither abnormal amyloid deposition nor obvious cell infiltration. The beneficial effects of O. biennis and H. perforatum for attenuating myelin loss and amyloid deposition suggest their therapeutic utility for treatment of MS.
我们研究了月见草和贯叶连翘提取物对实验性自身免疫性脑脊髓炎（EAE）小鼠脑组织组织病理学、髓鞘少突胶质细胞糖蛋白（MOG）、髓鞘碱性蛋白（MBP）、总抗氧化状态（TAS）、总氧化状态（TOS）和氧化应激指数（OSI）的影响。47只C57BL/6J小鼠被分为以下几组：多发性硬化症（MS）组、对照组（健康小鼠）、MS + 贯叶连翘治疗组（MS + HP）、MS + 月见草治疗组（MS + OB）。除对照组外，所有组均采用EAE方法进行免疫。免疫两周后，MS + HP组的小鼠喂食含18 - 21 g/kg贯叶连翘提取物的正常食物，MS + OB组的小鼠喂食含18 - 21 g/kg月见草提取物的正常食物，对照组和MS组的小鼠喂食正常食物六周。从所有小鼠收集脑组织样本进行组织病理学和生化分析。每天使用功能系统评分（FSS）对疾病的临床症状进行评分。贯叶连翘和月见草提取物改善了MS动物脑组织中升高的MOG和MBP值。贯叶连翘和月见草提取物降低了脑组织的TOS和OSI值，并提高了MS动物脑组织中的TAS水平。此外，与提取物给药开始时相比，贯叶连翘和月见草提取物在实验结束时降低了临床症状。我们发现MS组与对照组相比髓鞘丢失。贯叶连翘和月见草提取物治疗减少了MS + HP组和MS + OB组的髓鞘丢失量。我们还观察到MS组血管壁、神经元细胞质和细胞间隙中有淀粉样沉积。月见草和贯叶连翘治疗组既没有异常的淀粉样沉积也没有明显的细胞浸润。月见草和贯叶连翘对减轻髓鞘丢失和淀粉样沉积的有益作用表明它们在治疗MS方面的治疗效用。
