Department of Biochemistry and Applied Chemistry, National Institute of Technology, Kurume College, 1-1-1 Komorino, Kurume 830-8555, Japan.
Photochem Photobiol Sci. 2019 Jun 12;18(6):1447-1460. doi: 10.1039/c8pp00593a.
Fluorescence change systems that can respond to biological objects have attracted attention for use as biological probes and sensors. In this study, we report emission enhancement in a fluorescent aggregate composed of amphiphilic donor-acceptor dye molecules. The emission efficiency of the aggregate was reduced upon introducing a hydrophilic galactopyranose moiety, because of the decrease in the aggregate stability, which in turn was due to disruption of the hydrophilicity-hydrophobicity balance. In contrast, emission enhancement could be achieved by treatment with β-galactosidase, as a result of the removal of the galactopyranose moiety. The change in aggregate stabilization based on the hydrophilicity-hydrophobicity balance leads to the emission enhancement into detectable β-galactosidase activity.
荧光变化系统可以响应生物客体，因此作为生物探针和传感器受到关注。在这项研究中，我们报告了由两亲性给体-受体染料分子组成的荧光聚集体中的发射增强。由于聚集体稳定性的降低，导致亲水性-疏水性平衡被破坏，从而导致聚集体的发射效率降低。相反，通过用β-半乳糖苷酶处理，可以实现发射增强，这是由于去除了半乳糖吡喃糖基部分的结果。基于亲水性-疏水性平衡的聚集体稳定性变化导致发射增强，从而可以检测到β-半乳糖苷酶的活性。
