Waku Tomonori, Hirata Naoyuki, Nozaki Masamichi, Nogami Kanta, Kunugi Shigeru, Tanaka Naoki
Faculty of Molecular Chemistry and Engineering, Kyoto Institute of Technology, Gosyokaido-cho, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
Polymers (Basel). 2018 Dec 28;11(1):39. doi: 10.3390/polym11010039.
Morphological control of nanostructures that are composed of amphiphilic di- or tri-block molecules by external stimuli broadens their applications for molecular containers, nanoreactors, and controlled release materials. In this study, triblock amphiphiles comprising oligo(ethylene glycol), oligo(l-lysine), and tetra(l-phenylalanine) were prepared for the construction of nanostructures that can transform accompanying α-to-β transition of core-forming peptides. Circular dichroic (CD) measurements showed that the triblock amphiphiles adopted different secondary structures depending on the solvent environment: they adopt β-sheet structures in aqueous solution, while α-helix structures in 25% 2,2,2-trifluoroethanol (TFE) solution under basic pH conditions. Transmission electron microscopic (TEM) observation revealed that the triblock amphiphiles formed vesicle structures in 25% TFE aq. Solvent exchange from 25% TFE to water induced morphological transformation from vesicles to arc-shaped nanostructures accompanying α-β conformational transition. The transformable nanostructures may be useful as novel smart nanomaterials for molecular containers and micro reactors.
通过外部刺激对由两亲性二嵌段或三嵌段分子组成的纳米结构进行形态控制，拓宽了它们在分子容器、纳米反应器和控释材料方面的应用。在本研究中，制备了包含聚乙二醇、聚（L-赖氨酸）和四（L-苯丙氨酸）的三嵌段两亲分子，用于构建可伴随成核肽的α到β转变而转变的纳米结构。圆二色性（CD）测量表明，三嵌段两亲分子根据溶剂环境采用不同的二级结构：它们在水溶液中采用β-折叠结构，而在碱性pH条件下于25% 2,2,2-三氟乙醇（TFE）溶液中采用α-螺旋结构。透射电子显微镜（TEM）观察显示，三嵌段两亲分子在25% TFE水溶液中形成囊泡结构。从25% TFE到水的溶剂交换诱导了伴随α-β构象转变的从囊泡到弧形纳米结构的形态转变。这种可转变的纳米结构可能作为用于分子容器和微反应器的新型智能纳米材料有用。
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