Ziąbka Magdalena, Dziadek Michał, Królicka Aleksandra
AGH University of Science and Technology, Faculty of Materials Science and Ceramics, Department of Ceramics and Refractories, 30-059 Krakow, Poland.
AGH University of Science and Technology, Faculty of Materials Science and Ceramics, Department of Glass Technology and Amorphous Coatings, 30-059 Krakow, Poland.
Polymers (Basel). 2019 Jan 6;11(1):79. doi: 10.3390/polym11010079.
Polymers modified with bioactive nanoparticles are a promising solution for patients who need a tissue replacement. Modern implants, thanks to bioactive and bactericidal functions, facilitate the healing and regeneration process of the replaced tissue. The aim of this study was to assess whether silver nanoparticles (AgNPs) could support antibacterial function without cytotoxic effect and deterioration of biostability. This article describes biological and physiochemical aspects concerning a new polymeric middle ear implant (Otoimplant) enriched with silver nanoparticles. This kind of prosthesis is a promising implant for the reconstruction of ossicles in ossiculoplasty. We found that incorporation of silver nanoparticles into a polymeric matrix resulted in bactericidal efficacy against Gram-positive and Gram-negative bacteria, both resistant to antibiotics and basic strains. Our prostheses do not show cytotoxic effect and are a suitable biomaterial platform for effective culture of Saos2 and NHOst osteoblastic cells. The in vitro incubation of the samples in distilled water revealed that surface parameters, such as roughness, may slightly increase as a result of unveiling nanoparticles. However, the prolonged immersion does not change mechanical parameters. During one-year incubation, the prosthesis proved to retain stable values of Young's modulus, tensile strength, propagation of longitudinal ultrasonic waves, pH, and conductivity.
用生物活性纳米颗粒改性的聚合物对于需要组织替代物的患者来说是一种很有前景的解决方案。现代植入物由于具有生物活性和杀菌功能，有助于被替代组织的愈合和再生过程。本研究的目的是评估银纳米颗粒（AgNPs）是否能够在不产生细胞毒性作用和不降低生物稳定性的情况下支持抗菌功能。本文描述了一种富含银纳米颗粒的新型聚合物中耳植入物（耳植入物）的生物学和物理化学方面。这种假体是鼓室成形术中用于听小骨重建的一种很有前景的植入物。我们发现，将银纳米颗粒掺入聚合物基质中可产生针对革兰氏阳性和革兰氏阴性细菌的杀菌效果，这些细菌既有抗生素耐药菌，也有基本菌株。我们的假体没有显示出细胞毒性作用，并且是用于Saos2和NHOst成骨细胞有效培养的合适生物材料平台。将样品在蒸馏水中进行体外孵育发现，由于纳米颗粒暴露，表面参数（如粗糙度）可能会略有增加。然而，长时间浸泡不会改变机械参数。在一年的孵育过程中，该假体被证明能保持杨氏模量、拉伸强度、纵向超声波传播、pH值和电导率的稳定值。
