AGH University of Science and Technology, Faculty of Materials Science and Ceramics, Department of Ceramics and Refractories, Krakow 30-059, Poland.
AGH University of Science and Technology, Faculty of Materials Science and Ceramics, Department of Glass Technology and Amorphous Coatings, Krakow 30-059, Poland.
Molecules. 2017 Oct 10;22(10):1681. doi: 10.3390/molecules22101681.
Materials used in ossicular replacement prostheses must possess appropriate biological properties, such as biocompatibility, stability, no cytotoxicity. Due to the risk of infection (otitis media and chronic otitis media), it is desirable to use an antibacterial agent for illness prevention during the ossicular reconstruction. The goal of this work was to observe biological properties of a new composite prosthesis made of ABS containing silver nanoparticles (AgNPs 45T). Samples for biological tests and then a prototype of middle ear prosthesis were prepared using injection moulding and extrusion techniques. In vitro experiments were carried out to assess bactericidal efficacy against and standard strains, cell proliferation, viability and cytotoxicity, using Hs680.Tr. fibroblast cells. Surface parameters of the samples were evaluated, including roughness and wettability. The silver ions were continually released from the polymer in aqueous solution. The silver ions release was measured as increasing with time and concentration of the silver nanoparticles in the polymer matrix. No cytotoxicity effect was observed, while bactericidal efficacy was noticed for silver nanoparticles. The roughness studies showed an increase in roughness for the samples with silver nanoparticles. All polymer and composite materials containing silver nanoparticles showed hydrophilic properties. The composites were found to release silver ions at a concentration level capable of rendering the antimicrobial efficacy even with the lowest concentration of silver nanoparticles in the material. Our results demonstrate that middle ear prosthesis made of polymer and silver nanoparticles may eliminate bacteria during inflammation in the middle ear.
用于听骨重建的材料必须具有适当的生物学特性，如生物相容性、稳定性、无细胞毒性。由于感染的风险（中耳炎和慢性中耳炎），在听骨重建期间使用抗菌剂来预防疾病是可取的。这项工作的目的是观察一种新型复合材料人工听小骨的生物学特性，该复合材料由含有银纳米颗粒（AgNPs45T）的 ABS 制成。使用注塑和挤出技术为生物测试准备了样本，然后为中耳假体制作了原型。进行了体外实验来评估对 和 标准菌株的杀菌效果、细胞增殖、活力和细胞毒性，使用 Hs680.Tr.成纤维细胞。评估了样品的表面参数，包括粗糙度和润湿性。银离子在水溶液中持续从聚合物中释放出来。随着聚合物中银纳米颗粒的时间和浓度的增加，测量到银离子的释放增加。未观察到细胞毒性作用，而观察到银纳米颗粒的杀菌效果。粗糙度研究表明，含银纳米颗粒的样品的粗糙度增加。所有含有银纳米颗粒的聚合物和复合材料均表现出亲水性。研究发现，复合材料以能够达到抗菌效果的浓度释放银离子，即使材料中银纳米颗粒的浓度最低。我们的结果表明，由聚合物和银纳米颗粒制成的中耳假体可以在中耳炎症期间消除细菌。
