School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001, China.
J Nanosci Nanotechnol. 2019 Sep 1;19(9):5463-5468. doi: 10.1166/jnn.2019.16521.
The purpose of current research is to develop ultrasound-triggered gas-generating Doxorubicin PLGA nanoparticle for cancer therapy. Method: pH-sensitive PLGA nanoparticles (PLGANPs) was fabricated to deliver doxorubicin (DOX) and sodium bicarbonate (NaHCO₃) using the water-in-oil-in-water (w/o/w) double emulsion method. Result: The nanoparticle with the size (650 nm) and high drug loading (15.8±2.3%) were successfully prepared and showed pH-responsive release characteristics. results indicate that DOX/NaHCO₃@PLGANPs with ultrasound had higher inhibition and cell uptake on MCF-7 cells than free DOX and other formulation. animal experiments showed that after treatment of DOX/NaHCO₃@PLGANPs with ultrasound, the relative tumor volume (0.63) of S180-tumor-bearing mice was lower than that of without ultrasound (0.81), DOX@PLGANPs (1.00) and Free DOX (1.12). Moreover, safety evaluation result indicated that DOX/NaHCO₃@PLGANPs was safer than free DOX. In conclusion, the DOX/NaHCO₃@PLGANPs was successfully developed and evaluated and . This drug delivery system will be a promising strategy for cancer therapy and diagnosis.
本研究旨在开发超声触发的载多柔比星聚乳酸-羟基乙酸纳米粒用于癌症治疗。方法：采用水包油包水（w/o/w）复乳法制备 pH 敏感的载多柔比星聚乳酸-羟基乙酸纳米粒（PLGANPs），以递送多柔比星（DOX）和碳酸氢钠（NaHCO₃）。结果：成功制备了粒径（650nm）和高载药量（15.8±2.3%）的纳米粒，并表现出 pH 响应性释放特性。结果表明，超声处理后的 DOX/NaHCO₃@PLGANPs 对 MCF-7 细胞的抑制和摄取作用均高于游离 DOX 和其他制剂。动物实验表明，超声处理 DOX/NaHCO₃@PLGANPs 后，荷 S180 瘤小鼠的相对肿瘤体积（0.63）低于无超声（0.81）、DOX@PLGANPs（1.00）和游离 DOX（1.12）。此外，安全性评价结果表明，DOX/NaHCO₃@PLGANPs 比游离 DOX 更安全。总之，成功开发并评价了 DOX/NaHCO₃@PLGANPs，为癌症治疗和诊断提供了一种有前途的策略。
