tambaqui精液冷冻保存中谷胱甘肽和ATP的减少

Reduced Glutathione and ATP in the Seminal Cryopreservation of Tambaqui.

作者信息

Pereira F Alves, Corcini C Dahl, da Silva A Cardoso, Pires D Martins, Pereira J Ribeiro, Gheller S Mari Meneghello, Streit D Pedro, Maria A Nizio, Varela A Sergio

机构信息

Programa de pós graduação em Biologia de Ambientes Aquáticos Continentais, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, Rio Grande, RS, Brasil.

Reprodução Animal Comparada- RAC, Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, Rio Grande, RS, Brasil. ReproPel, Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, RS, Brasil.

出版信息

Cryo Letters. 2018 Nov/Dec;39(6):371-379.

PMID:30963154

Abstract

BACKGROUND

The aim of this study was to analyze the effect of reduced glutathione (GR) and adenosine triphosphate (ATP) in cryopreserved sperm tambaqui, Colossoma macropomum..

OBJECTIVE

Semen samples were frozen at different concentrations of GR (2, 4, and 6 mM) and ATP (7.5, 15, 30 mM) evaluated separately.

MATERIALS AND METHODS

Different concentrations of GR and ATP were added to the diluent Beltsville Thawing Solution 10 % of DMSO (extender control). The thawed sperm was evaluated as the kinetic parameters for Computer Assisted Semen Analysis (CASA) and cell feature parameters by flow cytometry.

RESULTS

All concentrations of GR and ATP had gradual reduction in the production of reactive oxygen species, mainly ATP 15; 22.5 mM and 30 mM, which decreased by 50% or more when compared to the control. All concentrations of GR and ATP did not change (P> 0.05) DNA fragmentation and lipid peroxidation. The membrane fluidity GR in concentrations 4 and 6 mM were better than control; and the concentrations of 2 and 4 mM maintained more efficiently to motility period, total and progressive motility.

CONCLUSION

Thus, it is indicated the use of BTS extender with 10% DMSO added 4 mM GR thawed sperm Tambaqui C. macropomum.

摘要

背景

本研究旨在分析还原型谷胱甘肽（GR）和三磷酸腺苷（ATP）对冷冻保存的巨脂鲤（Colossoma macropomum）精子的影响。

目的

分别评估在不同浓度的GR（2、4和6 mM）和ATP（7.5、15、30 mM）下冷冻的精液样本。

材料与方法

将不同浓度的GR和ATP添加到含有10%二甲基亚砜的Beltsville解冻溶液（稀释剂对照）中。解冻后的精子通过计算机辅助精液分析（CASA）评估其动力学参数，并通过流式细胞术评估细胞特征参数。

结果

所有浓度的GR和ATP均可使活性氧的产生逐渐减少，主要是ATP浓度为15、22.5 mM和30 mM时，与对照组相比减少了50%或更多。所有浓度的GR和ATP均未改变（P>0.05）DNA片段化和脂质过氧化。浓度为4和6 mM的GR的膜流动性优于对照组；浓度为2和4 mM时能更有效地维持活力期、总活力和渐进性活力。