Federal University of Rio Grande, Biology of Continental Aquatic Environments. Rio Grande, Brazil.
Federal University of Pelotas, Veterinary Medicine. Capao do Leao, Brazil.
Cryo Letters. 2020 Jan-Feb;41(1):13-18.
ATP exogenous (ATPe) has been used successfully in improving motility and fertility for many animal species. However this has not yet been tested on Brycon orbignyamus.
The objective of this study was to evaluate the use of ATPe for the cryopreservation of sperm from B. orbignyamus.
The ATPe concentrations tested were 1.0 µM, 5.0 µM and 10 µM combined with Beltsville Thawing Solution extender and dimethylformamide at 7.5%. The sperm were frozen in a nitrogen vapour vessel and stored in liquid nitrogen at -196 ºC. The parameters of viability post-thawing were evaluated using CASA, and flow cytometer.
The ATPe did not promote improvements in spermatic kinetics, and in the higher concentrations caused a worsening in these parameters. Also there was loss of mitochondrial functionality and greater cellular disruption with the concentration of 10 μM.
We do not recommend the addition of ATP for cryopreserving B. orbignyamus.
三磷酸腺苷（ATPe）已成功应用于提高许多动物物种的运动能力和生育能力。然而，这在 Brycon orbignyamus 上尚未得到测试。
本研究的目的是评估使用 ATPe 对 B. orbignyamus 精子进行冷冻保存的效果。
测试的 ATPe 浓度分别为 1.0µM、5.0µM 和 10µM，与 Beltsville 解冻液和 7.5%二甲基甲酰胺结合使用。精子在氮蒸气容器中冷冻，并在-196°C 的液氮中储存。使用 CASA 和流式细胞仪评估解冻后活力的参数。
ATPe 并未促进精子动力学的改善，并且在较高浓度下会导致这些参数恶化。此外，浓度为 10μM 时还会导致线粒体功能丧失和细胞损伤加剧。
我们不建议在冷冻保存 B. orbignyamus 时添加 ATP。
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