Department of Obstetrics and Gynecology, Hanchuan People's Hospital, Hanchuan, China.
Eur Rev Med Pharmacol Sci. 2019 Mar;23(6):2366-2373. doi: 10.26355/eurrev_201903_17381.
The aim of this study was to investigate the effect of microRNA-539-3p (miR-539-3p) on the development of epithelial ovarian cancer (EOC), and to explore the possible underlying mechanism.
A total of 40 paired EOC tissues and adjacent normal ovarian tissues were surgically resected in Hanchuan People's Hospital. Quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR) was used to detect the expression of miR-539-3p in EOC tissues and cell lines. Targeted regulatory mechanism of miR-539-3p on SPARC-like protein 1 (SPARCL1) was identified by luciferase reporter and Western blot assays. Furthermore, the effects of miR-539-3p/SPARCL1 axis on the malignant behaviors of EOC cells, including proliferation, invasion and migration abilities, were confirmed by cell counting kit-8 (CCK-8), transwell and scratch wound assays.
QRT-PCR showed that the expression of miR-539-3p was significantly up-regulated in EOC tissues and cell lines. SPARCL1 was a direct target of miR-539-3p in EOC cells. Overexpression of miR-539-3p significantly promoted the proliferation, migration and invasion of SKOV3 cells. Furthermore, co-transfection of miR-539-3p inhibitor and si-SPARCL1 could remarkably restore the migration and invasion abilities of SKOV3 cells.
MiR-539-3p acted as an oncogene in EOC by targeting SPARCL1. MiR-539-3p/SPARCL1 axis, as a target for the treatment of EOC, might become a feasible and new method of tumor treatment.
本研究旨在探讨微小 RNA-539-3p（miR-539-3p）对卵巢上皮性癌（EOC）发展的影响，并探讨其可能的潜在机制。
本研究共收集了汉川人民医院手术切除的 40 对 EOC 组织和相邻正常卵巢组织。采用定量逆转录-聚合酶链反应（qRT-PCR）检测 miR-539-3p 在 EOC 组织和细胞系中的表达。通过荧光素酶报告和 Western blot 检测 miR-539-3p 对 SPARC 样蛋白 1（SPARCL1）的靶向调控机制。进一步通过细胞计数试剂盒-8（CCK-8）、Transwell 和划痕实验，证实 miR-539-3p/SPARCL1 轴对 EOC 细胞恶性行为（包括增殖、侵袭和迁移能力）的影响。
qRT-PCR 结果显示，miR-539-3p 在 EOC 组织和细胞系中的表达明显上调。SPARCL1 是 EOC 细胞中 miR-539-3p 的直接靶标。过表达 miR-539-3p 显著促进 SKOV3 细胞的增殖、迁移和侵袭。此外，miR-539-3p 抑制剂和 si-SPARCL1 的共转染可显著恢复 SKOV3 细胞的迁移和侵袭能力。
miR-539-3p 通过靶向 SPARCL1 在 EOC 中发挥癌基因作用。miR-539-3p/SPARCL1 轴作为 EOC 的治疗靶点，可能成为一种可行的新的肿瘤治疗方法。
