Department of Obstetrics and Gynecology, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China.
Eur Rev Med Pharmacol Sci. 2019 Mar;23(6):2374-2379. doi: 10.26355/eurrev_201903_17382.
Recent studies have determined the crucial role of long noncoding RNAs (lncRNAs) in cancer development. Cervical cancer (CC) is a common type of fatal gynecological cancer worldwide. This study aims to identify the role of lncRNA AB073614 in the progression of CC.
Relative level of AB073614 in 3 CC cell lines and 48 paired CC samples was determined by the quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). The potential regulatory effects of AB073614 on the cellular behaviors of CC cells were explored through apoptosis assay, colony formation assay, and proliferation assay in vitro. The underlying mechanism of AB073614 in mediating the progression of CC was also conducted.
The AB073614 expression was remarkably higher in CC tissues than that in adjacent tissues. The knockdown of AB073614 inhibited proliferation but induced apoptosis of CC cells. In addition, RBM5 (RNA binding motif 5) was upregulated in CC cells after knockdown of AB073614 in vitro. Furthermore, a negative correlation was seen between the expressions of RBM5 and AB073614 in CC tissues.
AB073614 could promote proliferation and inhibit apoptosis of CC cells through downregulating RBM5, which may offer a new therapeutic intervention for CC patients.
最近的研究确定了长非编码 RNA（lncRNA）在癌症发展中的关键作用。宫颈癌（CC）是全球常见的致命妇科癌症。本研究旨在确定 lncRNA AB073614 在 CC 进展中的作用。
通过实时定量聚合酶链反应（qRT-PCR）测定 3 种 CC 细胞系和 48 对 CC 样本中 AB073614 的相对水平。通过体外凋亡试验、集落形成试验和增殖试验探讨 AB073614 对 CC 细胞生物学行为的潜在调节作用。还进行了 AB073614 介导 CC 进展的潜在机制研究。
AB073614 在 CC 组织中的表达明显高于相邻组织。AB073614 的敲低抑制了 CC 细胞的增殖，但诱导了其凋亡。此外，体外敲低 AB073614 后，CC 细胞中 RBM5（RNA 结合基序 5）上调。此外，CC 组织中 RBM5 和 AB073614 的表达呈负相关。
AB073614 可能通过下调 RBM5 促进 CC 细胞的增殖并抑制其凋亡，这可能为 CC 患者提供新的治疗干预措施。
