Department of Radiology, Weihai Municipal Hospital, The Affiliated Hospital of Binzhou Medical College, Weihai, Shandong, China.
Eur Rev Med Pharmacol Sci. 2019 Mar;23(6):2409-2416. doi: 10.26355/eurrev_201903_17387.
Our study aims to explore the correlations of magnetic resonance imaging (MRI) features and pathological changes in liver cancer with the messenger ribonucleic acid (mRNA) and protein expression of Beclin1.
The mRNA and protein expression levels of Beclin1 in 42 cases of liver cancer and para-carcinoma tissues from liver cancer patients were detected via fluorescence quantitative polymerase chain reaction (FQ-PCR) and immunohistochemistry, and its correlations with the clinicopathological characteristics and MRI features were analyzed.
The mRNA expression of Beclin1 in liver cancer tissues was significantly higher than that in para-carcinoma tissues, and statistical correlation between pathological grade and mass size of liver cancer was found (p < 0.05). The immunohistochemical results revealed that the Beclin1 positive expression was mainly located in the cytoplasm, and the level was significantly increased compared with that in corresponding para-carcinoma tissues (p < 0.05). The MRI showed that both Min-apparent diffusion coefficient (ADC) and Mean-ADC in Beclin1 high-expression group were significantly lower than those in Beclin1 low-expression group.
The Beclin1 expression plays an important role in the occurrence and development of liver cancer. MRI shows significant correlation with the level of Beclin1 in liver cancer, which provides certain value in the evaluation of the biological behaviors and prognosis of liver cancer.
本研究旨在探讨肝癌磁共振成像（MRI）特征与病理变化与 Beclin1 信使核糖核酸（mRNA）和蛋白表达的相关性。
通过荧光定量聚合酶链反应（FQ-PCR）和免疫组织化学检测 42 例肝癌及肝癌患者癌旁组织中 Beclin1 的 mRNA 和蛋白表达水平，并分析其与临床病理特征和 MRI 特征的相关性。
肝癌组织中 Beclin1 的 mRNA 表达明显高于癌旁组织，且与肝癌肿块大小和病理分级呈统计学相关（p<0.05）。免疫组化结果显示，Beclin1 阳性表达主要位于细胞质，与相应癌旁组织相比明显升高（p<0.05）。MRI 显示 Beclin1 高表达组的最小表观扩散系数（ADC）和平均 ADC 值均显著低于 Beclin1 低表达组。
Beclin1 的表达在肝癌的发生发展中起重要作用。MRI 与肝癌中 Beclin1 的水平有显著相关性，为评估肝癌的生物学行为和预后提供了一定的价值。
