College of Animal Science and Technology, Guangxi University, Nanning, Guangxi 530004, China.
The Animal Husbandry Research Institute of Guangxi Zhuang Autonomous Region, Nanning, Guangxi 530001, China.
Bioresour Technol. 2019 Aug;285:121315. doi: 10.1016/j.biortech.2019.121315. Epub 2019 Apr 2.
Investigating the dynamic changes in bacterial community composition during sugarcane top silage production starting in late March and finishing in late June (storage temperature: 20 to 35 °C) will advance our understanding of ensilage in hot ambient temperatures. The results showed that, the fermentation process was dominated by Leuconostocaceae (until d 5), followed by Lactobacillaceae (from d 5 to d 30), and finally Lactobacillaceae and Clostridium (from d 60 to d 90). As the fermentation process progressed, there was a significant increase in Lactobacillaceae abundance, and on d 60 there was a sharp increase in Clostridiaceae abundance. Spearman's correlation showed that, Lactococcus and Leuconostoc abundance were negatively correlated with acetate, propionate, butyrate, and ammonia-N levels. Clostridiaceae and Lactobacillaceae abundance were positively correlated with acetate, propionate, butyrate, and ammonia-N levels. The high moisture content (DM 24.31%) of sugarcane tops stored in hot ambient temperatures may result in butyric acid fermentation.
调查从 3 月底到 6 月底（储存温度：20 到 35°C）甘蔗顶青贮生产过程中细菌群落组成的动态变化，将有助于我们了解高温环境下的青贮。结果表明，发酵过程前期以明串珠菌科（直到第 5 天）为主，随后是乳杆菌科（第 5 天至第 30 天），最后是乳杆菌科和梭菌科（第 60 天至第 90 天）。随着发酵过程的进行，乳杆菌科的丰度显著增加，第 60 天时梭菌科的丰度急剧增加。Spearman 相关性分析表明，乳球菌和肠球菌的丰度与乙酸盐、丙酸盐、丁酸盐和氨态氮水平呈负相关。梭菌科和乳杆菌科的丰度与乙酸盐、丙酸盐、丁酸盐和氨态氮水平呈正相关。高温环境下储存的甘蔗顶具有较高的水分含量（DM 24.31%），可能导致丁酸发酵。
