Department of Surgical Oncology, Osaka City University Graduate School of Medicine, Asahimachi 1-4-3, Abenoku, Osaka, Japan.
Esophagus. 2019 Oct;16(4):333-344. doi: 10.1007/s10388-019-00669-w. Epub 2019 Apr 9.
Dendritic cells (DCs) are the most potent antigen-presenting cells to induce cytotoxic T lymphocytes in the tumor environment. After acquiring antigens, DCs undergo maturation and their expression of MHC and co-stimulation molecules are enhanced, along with lysosome-associated membrane glycoprotein 3 (LAMP-3), which is a specific marker of mature DCs. In general, mature DCs are usually considered to be immunostimulatory in the cancer microenvironment. In addition, it is known that tumor-infiltrating lymphocytes (TILs) are associated with a good prognosis in esophageal squamous cell carcinoma (ESCC). However, few studies have targeted the interaction between DCs and TILs in the local immunity of ESCC. We investigated the localization of mature DCs within ESCC tissue and their relationship to TILs as well as the clinical outcome.
We evaluated 80 ESCC patients who underwent surgical treatment without preoperative treatment, using immunohistochemistry with LAMP-3 and CD8.
The results showed that LAMP-3 DCs were predominantly observed in the peritumoral area. Intratumoral CD8 T cells were found to be associated with a favorable prognosis, and the number of infiltrating LAMP-3 DCs was correlated with the number of intratumoral CD8 T cells.
At the local tumor site, mature LAMP-3 DCs might be associated with increasing tumor infiltrating CD8 T cells.
树突状细胞（DCs）是在肿瘤微环境中诱导细胞毒性 T 淋巴细胞的最有效抗原呈递细胞。摄取抗原后，DCs 经历成熟，其 MHC 和共刺激分子的表达增强，同时溶酶体相关膜糖蛋白 3（LAMP-3）也增强，LAMP-3 是成熟 DC 的特异性标志物。通常，成熟的 DC 被认为在肿瘤微环境中具有免疫刺激性。此外，已知肿瘤浸润淋巴细胞（TILs）与食管鳞状细胞癌（ESCC）的良好预后相关。然而，很少有研究针对 ESCC 局部免疫中 DCs 和 TILs 之间的相互作用。我们研究了成熟 DCs 在 ESCC 组织中的定位及其与 TILs 的关系以及临床结局。
我们评估了 80 名接受手术治疗而未接受术前治疗的 ESCC 患者，使用 LAMP-3 和 CD8 的免疫组织化学进行评估。
结果表明，LAMP-3 DCs 主要存在于肿瘤周围区域。肿瘤内 CD8 T 细胞与良好的预后相关，浸润的 LAMP-3 DCs 数量与肿瘤内 CD8 T 细胞的数量相关。
在局部肿瘤部位，成熟的 LAMP-3 DCs 可能与肿瘤浸润 CD8 T 细胞的增加有关。
