Kumar Anoop, Gupta Meenakshi, Sharma Ruchika, Sharma Neelima
Department of Pharmaceutical Science and Technology, Birla Institute of Technology, Mesra, Ranchi -835215, Jharkhand, India.
Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research (NIPER-Raebareli), Lucknow (UP), India.
Endocr Metab Immune Disord Drug Targets. 2020;20(1):67-76. doi: 10.2174/1871530319666190410144540.
Deltamethrin (DLM) is a type 2 pyrethroid insecticide used in agriculture and home to control pests. However, emerging reports have indicated the immunotoxicity of DLM.
Thus, in the current investigation, we have checked the immune-protective role of quercetin in DLM-induced immunotoxicity by using in silico and in vitro techniques.
In silico results have shown good interaction of quercetin towards immune cell receptors (T & B cell receptors). The findings of in vitro studies indicated the decrease in oxidative stress which is elevated by DLM in concentration & time-dependent manner. The increased caspases-3 activity was decreased by treatment of quercetin. The apoptosis induced by DLM in thymus and spleen was suppressed only at higher concentration (50μg/ml) of quercetin. Finally, the phenotypic changes due to DLM were restored by quercetin.
Quercetin has strong binding affinity towards CD4, CD8 and CD28, CD45 receptors and protects the thymocytes and splenocytes against DLM-induced apoptotic signaling pathways.
溴氰菊酯（DLM）是一种用于农业和家庭害虫防治的2型拟除虫菊酯类杀虫剂。然而，新出现的报道表明了DLM的免疫毒性。
因此，在当前的研究中，我们通过计算机模拟和体外技术检测了槲皮素在DLM诱导的免疫毒性中的免疫保护作用。
计算机模拟结果显示槲皮素与免疫细胞受体（T细胞和B细胞受体）有良好的相互作用。体外研究结果表明，DLM以浓度和时间依赖性方式升高的氧化应激有所降低。槲皮素处理降低了升高的半胱天冬酶-3活性。只有在较高浓度（50μg/ml）的槲皮素作用下，DLM诱导的胸腺和脾脏细胞凋亡才受到抑制。最后，槲皮素恢复了由DLM引起的表型变化。
槲皮素对CD4、CD8和CD28、CD45受体具有很强的结合亲和力，并保护胸腺细胞和脾细胞免受DLM诱导的凋亡信号通路的影响。
