溴氰菊酯诱导的免疫毒性及其槲皮素的保护作用：一项实验研究。
Deltamethrin-Induced Immunotoxicity and its Protection by Quercetin: An Experimental Study.
作者信息
Kumar Anoop, Gupta Meenakshi, Sharma Ruchika, Sharma Neelima
机构信息
Department of Pharmaceutical Science and Technology, Birla Institute of Technology, Mesra, Ranchi -835215, Jharkhand, India.
Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research (NIPER-Raebareli), Lucknow (UP), India.
出版信息
Endocr Metab Immune Disord Drug Targets. 2020;20(1):67-76. doi: 10.2174/1871530319666190410144540.
BACKGROUND
Deltamethrin (DLM) is a type 2 pyrethroid insecticide used in agriculture and home to control pests. However, emerging reports have indicated the immunotoxicity of DLM.
OBJECTIVE
Thus, in the current investigation, we have checked the immune-protective role of quercetin in DLM-induced immunotoxicity by using in silico and in vitro techniques.
RESULTS
In silico results have shown good interaction of quercetin towards immune cell receptors (T & B cell receptors). The findings of in vitro studies indicated the decrease in oxidative stress which is elevated by DLM in concentration & time-dependent manner. The increased caspases-3 activity was decreased by treatment of quercetin. The apoptosis induced by DLM in thymus and spleen was suppressed only at higher concentration (50μg/ml) of quercetin. Finally, the phenotypic changes due to DLM were restored by quercetin.
CONCLUSION
Quercetin has strong binding affinity towards CD4, CD8 and CD28, CD45 receptors and protects the thymocytes and splenocytes against DLM-induced apoptotic signaling pathways.
背景
溴氰菊酯（DLM）是一种用于农业和家庭害虫防治的2型拟除虫菊酯类杀虫剂。然而，新出现的报道表明了DLM的免疫毒性。
目的
因此，在当前的研究中，我们通过计算机模拟和体外技术检测了槲皮素在DLM诱导的免疫毒性中的免疫保护作用。
结果
计算机模拟结果显示槲皮素与免疫细胞受体（T细胞和B细胞受体）有良好的相互作用。体外研究结果表明，DLM以浓度和时间依赖性方式升高的氧化应激有所降低。槲皮素处理降低了升高的半胱天冬酶-3活性。只有在较高浓度（50μg/ml）的槲皮素作用下，DLM诱导的胸腺和脾脏细胞凋亡才受到抑制。最后，槲皮素恢复了由DLM引起的表型变化。
结论
槲皮素对CD4、CD8和CD28、CD45受体具有很强的结合亲和力，并保护胸腺细胞和脾细胞免受DLM诱导的凋亡信号通路的影响。
Zotero 插件