Richert L, Beck J P, Shinitzky M
Immunol Lett. 1986 Nov 3;13(6):329-34. doi: 10.1016/0165-2478(86)90067-2.
When murine BALB/c splenocytes are pretreated for 2 h with different concentrations of 25-hydroxycholesterol (25-HC) or 7 beta-hydroxycholesterol (7-HC), washed and stimulated either with irradiated C57BL/6 splenocytes or with Con A, IL-2 secretion is inhibited in a dose-dependent way as well as the subsequent cell proliferation. Using the same treatment and stimulation conditions, IL-2 receptor appearance on human T lymphocytes, as characterized by anti-Tac antibody binding, is also inhibited in a dose-dependent way. In contrast, the hydrophilic derivative of 7 beta-hydroxycholesterol, the 3.7 bishemisuccinate sodium salt (7-HC BHS), did not influence any of the 3 tested parameters.
当用不同浓度的25-羟基胆固醇（25-HC）或7β-羟基胆固醇（7-HC）对小鼠BALB/c脾细胞预处理2小时，洗涤后用辐照的C57BL/6脾细胞或刀豆蛋白A刺激时，白细胞介素-2（IL-2）的分泌以及随后的细胞增殖均受到剂量依赖性抑制。在相同的处理和刺激条件下，以抗Tac抗体结合为特征的人T淋巴细胞上IL-2受体的出现也受到剂量依赖性抑制。相比之下，7β-羟基胆固醇的亲水性衍生物3,7-二琥珀酸半酯钠盐（7-HC BHS）对所测试的三个参数均无影响。
