Takahashi M, Asada K
J Biol Chem. 1986 Dec 25;261(36):16923-6.
The sizes of the Mn-binding sites in spinach thylakoids were estimated by target size analysis, assaying the membrane-bound Mn that was resistant to EDTA washing after radiation inactivation. The inactivation curve showed well the inactivation of two independent Mn-binding sites of different sizes: about two-thirds of the Mn coordinated to a binding site of 65 kDa, and the rest bound to a much smaller site of only about 3 kDa. In the large site, there was about 1 g atom of Mn/110 mol of chlorophyll in spinach thylakoids, which was constant in normally grown plants, although the Mn level in the small site depended on culture conditions. Thylakoids that had been incubated with hydroxylamine or in 0.8 M Tris lost Mn exclusively from the large binding site.
通过靶尺寸分析估计菠菜类囊体中锰结合位点的大小，检测辐射失活后对EDTA洗涤有抗性的膜结合锰。失活曲线很好地显示了两个不同大小的独立锰结合位点的失活情况：约三分之二的锰与一个65 kDa的结合位点配位，其余的则与一个仅约3 kDa的小得多的位点结合。在大位点中，菠菜类囊体中每110摩尔叶绿素约有1克原子的锰，在正常生长的植物中这一数值是恒定的，尽管小位点中的锰水平取决于培养条件。用羟胺孵育或在0.8 M Tris中孵育的类囊体仅从大结合位点丢失锰。
