Farrar W L, Birchenall-Sparks M C, Young H B
J Immunol. 1986 Dec 15;137(12):3836-40.
Interleukin 2 (IL 2) induces specific mRNA synthesis and secretion of an important immunoregulatory molecule, interferon-gamma (IFN-gamma). We have observed that treatment of an IL 2 independent murine T cell line, BUD-27, with IL 2, calcium ionophore A23187, or agents that activate phospholipid/Ca2+-dependent protein kinase C results in increased IFN-gamma mRNA transcription and release of anti-viral activity. These same agents each induced the subcellular redistribution of protein kinase C from cytosol to plasma membrane in both the BUD-27 cell line and its IL 2-dependent parent, CT6. Ionophore concentrations greater than 1 micron exhibited the most significant induction of IFN-gamma mRNA, which also correlated with the dose of ionophore, inducing translocation of protein kinase C. This correlation between increased mRNA levels and protein kinase C translocation suggests that a calcium-dependent event is involved in induction of IFN-gamma mRNA synthesis. Furthermore, the magnitude of the translocation of protein kinase C from cytosol to plasma membrane corresponded to the physiologic IL 2 dose-response for IFN-gamma secretion. The data suggest that the activation of protein kinase C and/or coordinate elevation of intracellular calcium may provide at least one mechanism of signal transduction for the regulation of IFN-gamma gene transcription.
白细胞介素2（IL-2）可诱导一种重要的免疫调节分子——γ干扰素（IFN-γ）的特异性mRNA合成及分泌。我们观察到，用IL-2、钙离子载体A23187或激活磷脂/Ca²⁺依赖性蛋白激酶C的试剂处理一种不依赖IL-2的小鼠T细胞系BUD-27，会导致IFN-γ mRNA转录增加及抗病毒活性释放。这些相同的试剂在BUD-27细胞系及其依赖IL-2的亲本细胞CT6中均诱导了蛋白激酶C从胞质溶胶向质膜的亚细胞重新分布。大于1微米的离子载体浓度对IFN-γ mRNA的诱导最为显著，这也与诱导蛋白激酶C易位的离子载体剂量相关。mRNA水平升高与蛋白激酶C易位之间的这种相关性表明，钙依赖性事件参与了IFN-γ mRNA合成的诱导。此外，蛋白激酶C从胞质溶胶向质膜易位的程度与IFN-γ分泌的生理性IL-2剂量反应相对应。数据表明，蛋白激酶C的激活和/或细胞内钙的协同升高可能为IFN-γ基因转录的调节提供至少一种信号转导机制。
