Penczek Adriane, Burster Timo
Department of Neurosurgery, Ulm University Medical Center, D-89081 Ulm, Germany.
Department of Biology, School of Science and Technology, Nazarbayev University, Astana 010000, Republic of Kazakhstan.
Biomed Rep. 2019 Apr;10(4):245-249. doi: 10.3892/br.2019.1198. Epub 2019 Mar 8.
The serine protease cathepsin G (CatG) is involved in numerous processes associated with the innate and adaptive immune system. During an immune response, neutrophils secrete CatG, which can bind to the cell surface of immune cells to provoke the proteolytic processing of cytokines and chemokines in order to stimulate lymphocytes. The present study analyzed peripheral blood mononuclear cells to characterize T cell populations in terms of their CatG content by flow cytometry. It was identified that CatG was exclusively present on the cell surface of a subset of T regulatory cells (Tregs), cluster of differentiation (CD) 39 Tregs, which expressed CatG in contrast to CD39 Tregs. Additionally, CatG was expressed on double positive CD4CD8 T cells, T helper (Th) 9 cells and Th22 cells, implicating CatG as a novel marker to distinguish certain T cell subsets.
丝氨酸蛋白酶组织蛋白酶G（CatG）参与了许多与先天性和适应性免疫系统相关的过程。在免疫反应期间，中性粒细胞分泌CatG，它可以与免疫细胞的细胞表面结合，引发细胞因子和趋化因子的蛋白水解过程，从而刺激淋巴细胞。本研究通过流式细胞术分析外周血单核细胞，以根据其CatG含量来表征T细胞群体。研究发现，CatG仅存在于一部分调节性T细胞（Tregs）即分化簇（CD）39 Tregs 的细胞表面，与CD39 Tregs 相比，这些细胞表达CatG。此外，CatG在双阳性CD4CD8 T细胞、辅助性T细胞（Th）9细胞和Th22细胞上表达，这表明CatG是区分某些T细胞亚群的一种新标志物。
