Franklyn J A, Wilson M, Davis J R, Ramsden D B, Docherty K, Sheppard M C
J Endocrinol. 1986 Oct;111(1):R1-2. doi: 10.1677/joe.0.111r001.
We have reported previously the effect of thyroid status in vivo on pituitary cytoplasmic concentrations of messenger RNA (mRNA) encoding the thyrotrophin (TSH) beta-subunit (Franklyn, Lynam, Docherty et al, 1985). Studies in vitro of the regulation of TSH beta gene transcription have been confined to thyrotrophic tumour cells. We now report the demonstration of TSH beta-subunit mRNA in non-tumorous rat pituitary cells in primary culture. Treatment of cells with thyrotrophin-releasing hormone (TRH) and with forskolin resulted in a marked increase in cellular concentration of TSH beta-mRNA. These results suggest that TRH exerts a direct effect on the pretranslational events involved in TSH synthesis and further that the adenylate cyclase system may be involved in the regulation of synthesis. We have thus described a novel system for the study of TSH beta-subunit gene expression in normal rat pituitary cells in vitro.
我们之前已经报道过体内甲状腺状态对编码促甲状腺激素（TSH）β亚基的信使核糖核酸（mRNA）在垂体细胞质中浓度的影响（富兰克林、莱纳姆、多彻蒂等人，1985年）。对TSHβ基因转录调控的体外研究一直局限于促甲状腺肿瘤细胞。我们现在报告在原代培养的非肿瘤性大鼠垂体细胞中TSHβ亚基mRNA的证实情况。用促甲状腺激素释放激素（TRH）和福斯高林处理细胞，导致TSHβ-mRNA的细胞浓度显著增加。这些结果表明，TRH对TSH合成中涉及的翻译前事件有直接影响，并且进一步表明腺苷酸环化酶系统可能参与合成的调控。因此，我们描述了一种用于体外研究正常大鼠垂体细胞中TSHβ亚基基因表达的新系统。
