Centre for Technology Alternatives for Rural Areas (CTARA), Indian Institute of Technology Bombay, Mumbai, Maharashtra, 400076, India.
Centre for Transportation Systems, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, 247667, India.
Environ Sci Pollut Res Int. 2019 Jun;26(16):16115-16124. doi: 10.1007/s11356-019-04946-0. Epub 2019 Apr 10.
The present study reports the use of Citrus limetta (CL) residue for cultivating Chlorella sp. mixotrophically to augment production of biodiesel. The cultivation of Chlorella sp. using CL as media was carried out by employing a fed-batch technique in open tray (open tray+CL) and in software (BioXpert V2)-attached automated photobioreactor (PBR+CL) systems. Data showed the limit of nitrogen substituent and satisfactory organic source of carbon (OSC) in CL, causing > 2-fold higher lipid content in cells, cultivated in both the systems than in control. For the cells grown in both the systems, ≥ 3-fold enhancement in lipid productivity was observed than in control. The total fatty acid methyl ester (FAME) concentrations from lipids extracted from cells grew in PBR+CL and in open tray+CL techniques were calculated as 50.59% and 38.31%, respectively. The PBR+CL system showed improved outcomes for lipid content, lipid and biomass productivity, FAME characteristics and physical property parameters of biodiesel than those obtained from the open tray+CL system. The physical property parameters of biodiesel produced from algal cells grown in PBR+CL were comparable to existing fuel standards. The results have shown lower cold filter plugging point (- 6.57 °C), higher cetane number (58.04) and average oxidative stability (3.60 h). Collectively, this investigation unveils the novel deployment of CL as a cost-effective feedstock for commercialisation of biodiesel production.
本研究报告了利用酸橙（CL）残渣混合培养小球藻来增加生物柴油产量。采用分批进料技术，在开放式托盘（open tray+CL）和软件（BioXpert V2）附着的自动化光生物反应器（PBR+CL）系统中，以 CL 作为培养基培养小球藻。数据显示，CL 中的氮取代限制和满意的有机碳源（OSC），导致细胞中的脂质含量比对照组高出 2 倍以上。对于在这两种系统中生长的细胞，脂质生产力的提高均高于对照组。从 PBR+CL 和 open tray+CL 技术中生长的细胞中提取的总脂肪酸甲酯（FAME）浓度分别计算为 50.59%和 38.31%。与 open tray+CL 系统相比，PBR+CL 系统在脂质含量、脂质和生物量生产力、FAME 特性和生物柴油物理性质参数方面显示出了更好的结果。从 PBR+CL 中生长的藻类细胞生产的生物柴油的物理性质参数与现有燃料标准相当。结果表明，冷滤堵塞点较低（-6.57°C），十六烷值较高（58.04），平均氧化稳定性较好（3.60 h）。总的来说，这项研究揭示了 CL 作为一种具有成本效益的生物柴油生产商业化原料的新应用。
