Sun Shuang, Liu Shimin, Luo Jun, Chen Zhi, Li Cong, Loor Juan J, Cao Yanhong
Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, China.
School of Agriculture and Environment, The University of Western Australia, Crawley, Western Australia, Australia.
Reprod Domest Anim. 2019 Jun;54(6):873-881. doi: 10.1111/rda.13439. Epub 2019 May 3.
This study aimed to elucidate the effects of repeated pregnant mare serum gonadotropin (PMSG) treatment for oestrous synchronization (ES) on ovarian gene expression and reproductive parameters in Xinong Saanen dairy goats, the dominant breed of dairy goat in China. The experiment was carried out at the Research Station of Northwest A&F University (NWAFU), China (34°16'N, 108°4'E). Forty-one does were randomly assigned to groups receiving ES treatments thrice every fortnight (3-PMSG group; n = 19), or ES treatment only once simultaneously with the third ES treatment in the 3-PMSG group (1-PMSG group; n = 22) during middle of the breeding season from late July (14 hr light) until late September (12 hr light). ES treatment was performed via intravaginal insertion of a controlled internal drug release (CIDR) device impregnated with 300 mg progesterone (P4), followed by 300 IU PMSG injections 48 hr before CIDR withdrawal. Oestrus was monitored using vasectomized bucks. Ovaries of three goats in oestrus from both groups were harvested for morphological examination and RNA sequencing (RNA-Seq). Then, all the oestrous goats in the 1-PMSG (n = 21) and 3-PMSG (n = 11) groups were artificially inseminated twice. The 3-PMSG group showed reduced oestrous rate (57.89%), pregnancy rate (31.58%) and litter size (1.17) compared, respectively, with 95.45%, 68.18% and 1.67 for 1-PMSG group (p < 0.05). However, no differences were found in the ovarian morphology between the 1-PMSG and 3-PMSG groups (p > 0.05). RNA-Seq revealed 114 differentially expressed genes (DEGs) in the ovaries of the 3-PMSG group, among which GCG, FSTL3, TET3 and AQP3 were deemed novel and promising candidate genes for regulating fertility. The present study indicates that the three-time PMSG treatment dysregulated several ovarian genes, thereby reducing reproductive performance.
本研究旨在阐明重复注射孕马血清促性腺激素（PMSG）进行发情同步化（ES）处理对中国主导奶山羊品种西农萨能奶山羊卵巢基因表达和繁殖参数的影响。实验在中国西北农林科技大学（NWAFU）试验站（北纬34°16′，东经108°4′）开展。在繁殖季节中期，即从7月下旬（光照14小时）至9月下旬（光照12小时），将41只母羊随机分为两组，一组每两周接受三次ES处理（3 - PMSG组；n = 19），另一组在3 - PMSG组第三次ES处理的同时仅接受一次ES处理（1 - PMSG组；n = 22）。ES处理通过阴道插入含300 mg孕酮（P4）的控释阴道装置（CIDR）进行，随后在取出CIDR前48小时注射300 IU PMSG。使用输精管结扎公羊监测发情情况。采集两组中处于发情期的三只山羊的卵巢进行形态学检查和RNA测序（RNA - Seq）。然后，对1 - PMSG组（n = 21）和3 - PMSG组（n = 11）所有发情母羊进行两次人工授精。与1 - PMSG组的95.45%、68.18%和1.67相比，3 - PMSG组的发情率（57.89%）、妊娠率（31.58%）和产仔数（1.17）均降低（p < 0.05）。然而，1 - PMSG组和3 - PMSG组之间的卵巢形态未发现差异（p > 0.05）。RNA - Seq显示3 - PMSG组卵巢中有114个差异表达基因（DEG），其中胰高血糖素原（GCG）、卵泡抑素样蛋白3（FSTL3）、TET甲基胞嘧啶双加氧酶3（TET3）和水通道蛋白3（AQP3）被认为是调节生育能力的新的且有前景的候选基因。本研究表明，三次PMSG处理使多个卵巢基因失调，从而降低了繁殖性能。
