魔术点核苷酸：可调靶标特异性化学酶合成。
Magic spot nucleotides: tunable target-specific chemoenzymatic synthesis.
机构信息
CIBSS - Centre for Integrative Biological Signalling Studies, University of Freiburg, 79104 Freiburg, Germany.
Institute of Pharmaceutical Biology, University of Freiburg, 79104 Freiburg, Germany.
出版信息
Chem Commun (Camb). 2019 May 10;55(37):5339-5342. doi: 10.1039/c9cc01688k. Epub 2019 Apr 11.
PMID:30973558
Abstract
A tunable chemoenzymatic strategy provides access to the entire class of magic spot nucleotides and modified analogues. The approach combines chemoselective bisphosphorylations using phosphoramidites with regioselective ribonuclease T2 cyclo-phosphate hydrolysis, leading to flexible and simple gram-scale operations.
摘要
一种可调的化学酶策略为获得整个魔点核苷酸和修饰类似物家族提供了途径。该方法结合了使用亚磷酰胺的化学选择性双磷酸化和区域选择性核糖核酸酶 T2 环磷酸水解，从而实现了灵活、简单的克级规模操作。
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