Department of Animal Physiology and Biochemistry, Kovalevsky Institute of Marine Biological Research Russian Academy of Sciences, Leninsky Ave 14, 119991, Moscow, Russian Federation.
Department of Animal Physiology and Biochemistry, Kovalevsky Institute of Marine Biological Research Russian Academy of Sciences, Leninsky Ave 14, 119991, Moscow, Russian Federation.
Fish Shellfish Immunol. 2019 Jun;89:361-367. doi: 10.1016/j.fsi.2019.04.017. Epub 2019 Apr 8.
The circulating hemocytes of cultivated marine mussel (Mytilus galloprovincialis) were investigated using light microscopy and flow cytometry. In mussels two cell types, granulocytes and agranulocytes, were identified based on the existence of two subpopulations of cells differing by size and granularity level on light-scattered plots. Light microscopic observation confirmed the presence of cells with cytoplasmic granules and cells without granulation in hemolymph of mussels. The main type of cells in hemolymph were agranular cells amounting 78.4 ± 8.9% in mussels. Flow cytometry showed that the agranular hemocytes of the mollusks produce significantly less reactive oxygen species compared to granulocytes. Mussel were exposed for 24 h of hypoxia and immune functions including hemocyte mortality, proliferation and reactive oxygen species (ROS) production were analysed using flow cytometric methods. Granulocyte number was higher at low oxygen concentration than that at normoxia; agranulocytes number decreased, in contrast. The ROS production after hypoxic treatment was decreased compared to normoxia level. No significant changes in hemocyte mortality and proliferation were observed.
利用光学显微镜和流式细胞术研究了养殖海洋贻贝（Mytilus galloprovincialis）的循环血细胞。根据光散射图上大小和颗粒度水平存在的两个细胞亚群，贻贝中鉴定出两种细胞类型，即粒细胞和无粒细胞。光学显微镜观察证实了贻贝血淋巴中存在具有细胞质颗粒的细胞和无颗粒的细胞。血淋巴中的主要细胞类型是无颗粒细胞，占贻贝的 78.4±8.9%。流式细胞术显示，与粒细胞相比，无颗粒血细胞产生的活性氧明显较少。贻贝在缺氧条件下暴露 24 小时，使用流式细胞术方法分析了免疫功能，包括血细胞死亡率、增殖和活性氧（ROS）产生。与正常氧浓度相比，低氧浓度下的粒细胞数量更高；相反，无粒细胞数量减少。与正常水平相比，缺氧处理后的 ROS 产生减少。血细胞死亡率和增殖没有明显变化。
